Oak protein profile alterations upon root colonization by an ectomycorrhizal fungus

Mónica Sebastiana, Joana Martins, Andreia Figueiredo, Filipa Monteiro, Jordi Sardans, Josep Peñuelas, Anabela Silva, Peter Roepstorff, Maria Salomé Pais, Ana Varela Coelho

    Research output: Contribution to journalArticleResearchpeer-review

    26 Citations (Scopus)


    © 2016, Springer-Verlag Berlin Heidelberg. An increased knowledge on the real impacts of ectomycorrhizal symbiosis in forest species is needed to optimize forest sustainable productivity and thus to improve forest services and their capacity to act as carbon sinks. In this study, we investigated the response of an oak species to ectomycorrhizae formation using a proteomics approach complemented by biochemical analysis of carbohydrate levels. Comparative proteome analysis between mycorrhizal and nonmycorrhizal cork oak plants revealed no differences at the foliar level. However, the protein profile of 34 unique oak proteins was altered in the roots. Consistent with the results of the biochemical analysis, the proteome analysis of the mycorrhizal roots suggests a decreasing utilization of sucrose for the metabolic activity of mycorrhizal roots which is consistent with an increased allocation of carbohydrates from the plant to the fungus in order to sustain the symbiosis. In addition, a promotion of protein unfolding mechanisms, attenuation of defense reactions, increased nutrient mobilization from the plant-fungus interface (N and P), as well as cytoskeleton rearrangements and induction of plant cell wall loosening for fungal root accommodation in colonized roots are also suggested by the results. The suggested improvement in root capacity to take up nutrients accompanied by an increase of root biomass without apparent changes in aboveground biomass strongly re-enforces the potential of mycorrhizal inoculation to improve cork oak forest resistance capacity to cope with coming climate change.
    Original languageEnglish
    Pages (from-to)109-128
    Issue number2
    Publication statusPublished - 1 Feb 2017


    • Cork oak
    • Differential in gel electrophoresis (DIGE)
    • Ectomycorrhizae
    • Mass spectrometry
    • Proteome
    • Symbiosis


    Dive into the research topics of 'Oak protein profile alterations upon root colonization by an ectomycorrhizal fungus'. Together they form a unique fingerprint.

    Cite this