Nutrient supply affects the mRNA expression profile of the porcine skeletal muscle

Tainã Figueiredo Cardoso, Raquel Quintanilla, Joan Tibau, Marta Gil, Emilio Mármol-Sánchez, Olga González-Rodríguez, Rayner González-Prendes, Marcel Amills

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)

Abstract

BACKGROUND: The genetic basis of muscle fat deposition in pigs is not well known. So far, we have only identified a limited number of genes involved in the absorption, transport, storage and catabolism of lipids. Such information is crucial to interpret, from a biological perspective, the results of genome-wide association analyses for intramuscular fat content and composition traits. Herewith, we have investigated how the ingestion of food changes gene expression in the gluteus medius muscle of Duroc pigs.RESULTS: By comparing the muscle mRNA expression of fasted pigs (T0) with that of pigs sampled 5 h (T1) and 7 h (T2) after food intake, we have detected differential expression (DE) for 148 (T0-T1), 520 (T0-T2) and 135 (T1-T2) genes (q-value <0.05 and a |FC| > of 1.5). Many of these DE genes were transcription factors, suggesting that we have detected the coordinated response of the skeletal muscle to nutrient supply. We also found DE genes with a dual role in oxidative stress and angiogenesis (THBS1, THBS2 and TXNIP), two biological processes that are probably activated in the post-prandial state. Finally, we have identified several loci playing a key role in the modulation of circadian rhythms (ARNTL, PER1, PER2, BHLHE40, NR1D1, SIK1, CIART and CRY2), a result that indicates that the porcine muscle circadian clock is modulated by nutrition.CONCLUSION: We have shown that hundreds of genes change their expression in the porcine skeletal muscle in response to nutrient intake. Many of these loci do not have a known metabolic role, a result that suggests that our knowledge about the genetic basis of muscle energy homeostasis is still incomplete.
Original languageEnglish
Pages (from-to)603
JournalBMC Genomics
Volume18
DOIs
Publication statusPublished - 10 Aug 2017

Keywords

  • Angiogenesis
  • Circadian rhythm
  • Oxidative stress
  • Pig
  • RNA-seq
  • Transcription factor

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