Novel strategy for sulfapyridine detection using a fully integrated electrochemical Bio-MEMS: Application to honey analysis

Nadia El Alami El Hassani, Abdoullatif Baraket, Ernandes Taveira Tenório Neto, Michael Lee, J. P. Salvador, M. P. Marco, Joan Bausells, Nezha El Bari, Benachir Bouchikhi, Abdelhamid Elaissari, Abdelhamid Errachid, Nadia Zine

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

© 2016 Sulfapyridine (SPy) is a sulfonamide antibiotic largely employed as veterinary drugs for prophylactic and therapeutic purposes. Therefore, its spread in the food products has to be restricted. Herein, we report the synthesis and characterization of a novel electrochemical biosensor based on gold microelectrodes modified with a new structure of magnetic nanoparticles (MNPs) coated with poly(pyrrole-co-pyrrole-2-carboxylic acid) (Py/Py-COOH) for high efficient detection of SPy. This analyte was quantified through a competitive detection procedure with 5-[4-(amino)phenylsulfonamide]-5-oxopentanoic acid-BSA (SA2-BSA) antigens toward polyclonal antibody (Ab-155). Initially, gold working electrodes (WEs) of integrated biomicro electro-mechanical system (BioMEMS) were functionalized by Ppy-COOH/MNPs, using a chronoamperometric (CA) electrodeposition. Afterward, SA2-BSA was covalently bonded to Py/Py-COOH/MNP modified gold WEs through amide bonding. The competitive detection of the analyte was made by a mixture of a fixed concentration of Ab-155 and decreasing concentrations of SPy from 50 µg L−1 to 2 ng L−1. Atomic Force Microscopy characterization was performed in order to ensure Ppy-COOH/MNPs electrodeposition on the microelectrode surfaces. Electrochemical measurements of SPy detection were carried out using electrochemical impedance spectroscopy (EIS). This biosensor was found to be highly sensitive and specific for SPy, with a limit of detection of 0.4 ng L−1. This technique was exploited to detect SPy in honey samples by using the standard addition method. The measurements were highly reproducible for detection and interferences namely, sulfadiazine (SDz), sulfathiazole (STz) and sulfamerazine (SMz). Taking these advantages of sensitivity, specificity, and low cost, our system provides a new horizon for development of advanced immunoassays in industrial food control.
Original languageEnglish
Pages (from-to)282-288
JournalBiosensors and Bioelectronics
Volume93
DOIs
Publication statusPublished - 15 Jul 2017

Keywords

  • BioMEMS
  • Biosensor
  • Food control
  • Magnetic nanoparticles (MNP)
  • Sulfapyridine

Fingerprint Dive into the research topics of 'Novel strategy for sulfapyridine detection using a fully integrated electrochemical Bio-MEMS: Application to honey analysis'. Together they form a unique fingerprint.

Cite this