Novel LTCC-potentiometric microfluidic device for biparametric analysis of organic compounds carrying plastic antibodies as ionophores: Application to sulfamethoxazole and trimethoprim

S. A.A. Almeida, E. Arasa, M. Puyol, C. S. Martinez-Cisneros, J. Alonso-Chamarro, M. C.B.S.M. Montenegro, M. G.F. Sales

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24 Citations (Scopus)

Abstract

Monitoring organic environmental contaminants is of crucial importance to ensure public health. This requires simple, portable and robust devices to carry out on-site analysis. For this purpose, a low-temperature co-fired ceramics (LTCC) microfluidic potentiometric device (LTCC/μPOT) was developed for the first time for an organic compound: sulfamethoxazole (SMX).Sensory materials relied on newly designed plastic antibodies. Sol-gel, self-assembling monolayer and molecular-imprinting techniques were merged for this purpose. Silica beads were amine-modified and linked to SMX via glutaraldehyde modification. Condensation polymerization was conducted around SMX to fill the vacant spaces. SMX was removed after, leaving behind imprinted sites of complementary shape. The obtained particles were used as ionophores in plasticized PVC membranes. The most suitable membrane composition was selected in steady-state assays. Its suitability to flow analysis was verified in flow-injection studies with regular tubular electrodes.The LTCC/μPOT device integrated a bidimensional mixer, an embedded reference electrode based on Ag/AgCl and an Ag-based contact screen-printed under a micromachined cavity of 600μm depth. The sensing membranes were deposited over this contact and acted as indicating electrodes. Under optimum conditions, the SMX sensor displayed slopes of about -58.7. mV/decade in a range from 12.7 to 250μg/mL, providing a detection limit of 3.85μg/mL and a sampling throughput of 36. samples/h with a reagent consumption of 3.3. mL per sample.The system was adjusted later to multiple analyte detection by including a second potentiometric cell on the LTCC/μPOT device. No additional reference electrode was required. This concept was applied to Trimethoprim (TMP), always administered concomitantly with sulphonamide drugs, and tested in fish-farming waters. The biparametric microanalyzer displayed Nernstian behaviour, with average slopes -54.7 (SMX) and +57.8. (TMP). mV/decade. To demonstrate the microanalyzer capabilities for real applications, it was successfully applied to single and simultaneous determination of SMX and TMP in aquaculture waters. © 2011 Elsevier B.V.
Original languageEnglish
Pages (from-to)197-203
JournalBiosensors and Bioelectronics
Volume30
Issue number1
DOIs
Publication statusPublished - 15 Dec 2011

Keywords

  • Biparametric analysis
  • Ceramic tapes
  • Miniaturization
  • Potentiometry
  • Sulfamethoxazole
  • Trimethoprim

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