Novel identification of peripheral dopaminergic D2 receptor in male germ cells

Joan Enric Rodríguez-Gil; Carola Otth; Mauricio Torres; Alfredo Ramírez; Juan Carlos Fernandez; Maite Castro; M. Cecilia Rauch; Mónica Brito; Alejandro J. Yañez; Juan Carlos Slebe; Ilona I. Concha

doi:https://doi.org/10.1002/jcb.21037

Novel identification of peripheral dopaminergic D2 receptor in male germ cells

Joan Enric Rodríguez-Gil, Carola Otth, Mauricio Torres, Alfredo Ramírez, Juan Carlos Fernandez, Maite Castro, M. Cecilia Rauch, Mónica Brito, Alejandro J. Yañez, Juan Carlos Slebe, Ilona I. Concha

Research output: Contribution to journal › Article › Research › peer-review

28 Citations (Scopus)

Abstract

Dopamine is a recognized modulator in the central nervous system (CNS) and peripheral organ functions. The presence of peripheral dopamine receptors outside the CNS has suggested an intriguing interaction between the nervous system and other functional systems, such as the reproductive system. In the present study we analyzed the expression of D2R receptors in rat testis, rat spermatogenic cells and spermatozoa, in different mammals. The RT-PCR analysis of rat testis mRNA showed specific bands corresponding to the two dopamine receptor D2R (L and S) isoforms previously described in the brain. Using Western blot analysis, we confirmed that the protein is present in rat testis, isolated spermatogenic cells and also in spermatozoa of a range of different mammals, such as rat, mouse, bull, and human. The immunohistochemistry analysis of rat adult testis showed that the receptor was expressed in all germ cells (pre- and post-meiotic phase) of the tubule with staining predominant in spermatogonia. Confocal analysis by indirect immunofluorescence revealed that in non-capacitated spermatozoa of rat, mouse, bull, and human, D2R is mainly localized in the flagellum, and is also observed in the acrosomal region of the sperm head (except in human spermatozoa). Our findings demonstrate that the two D2 receptor isoforms are expressed in rat testis and that the receptor protein is present in different mammalian spermatozoa. The presence of D2R receptors in male germ cells implies new and unsuspected roles for dopamine signaling in testicular and sperm physiology. © 2006 Wiley-Liss, Inc.

Original language	English
Pages (from-to)	141-150
Journal	Journal of Cellular Biochemistry
Volume	100
DOIs	https://doi.org/10.1002/jcb.21037
Publication status	Published - 1 Jan 2007

Keywords

D2 receptors
Male germ cells
Spermatozoa

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Rodríguez-Gil, J. E., Otth, C., Torres, M., Ramírez, A., Fernandez, J. C., Castro, M., Rauch, M. C., Brito, M., Yañez, A. J., Slebe, J. C., & Concha, I. I. (2007). Novel identification of peripheral dopaminergic D2 receptor in male germ cells. Journal of Cellular Biochemistry, 100, 141-150. https://doi.org/10.1002/jcb.21037

Rodríguez-Gil, Joan Enric ; Otth, Carola ; Torres, Mauricio ; Ramírez, Alfredo ; Fernandez, Juan Carlos ; Castro, Maite ; Rauch, M. Cecilia ; Brito, Mónica ; Yañez, Alejandro J. ; Slebe, Juan Carlos ; Concha, Ilona I. / Novel identification of peripheral dopaminergic D2 receptor in male germ cells. In: Journal of Cellular Biochemistry. 2007 ; Vol. 100. pp. 141-150.

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abstract = "Dopamine is a recognized modulator in the central nervous system (CNS) and peripheral organ functions. The presence of peripheral dopamine receptors outside the CNS has suggested an intriguing interaction between the nervous system and other functional systems, such as the reproductive system. In the present study we analyzed the expression of D2R receptors in rat testis, rat spermatogenic cells and spermatozoa, in different mammals. The RT-PCR analysis of rat testis mRNA showed specific bands corresponding to the two dopamine receptor D2R (L and S) isoforms previously described in the brain. Using Western blot analysis, we confirmed that the protein is present in rat testis, isolated spermatogenic cells and also in spermatozoa of a range of different mammals, such as rat, mouse, bull, and human. The immunohistochemistry analysis of rat adult testis showed that the receptor was expressed in all germ cells (pre- and post-meiotic phase) of the tubule with staining predominant in spermatogonia. Confocal analysis by indirect immunofluorescence revealed that in non-capacitated spermatozoa of rat, mouse, bull, and human, D2R is mainly localized in the flagellum, and is also observed in the acrosomal region of the sperm head (except in human spermatozoa). Our findings demonstrate that the two D2 receptor isoforms are expressed in rat testis and that the receptor protein is present in different mammalian spermatozoa. The presence of D2R receptors in male germ cells implies new and unsuspected roles for dopamine signaling in testicular and sperm physiology. {\textcopyright} 2006 Wiley-Liss, Inc.",

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Novel identification of peripheral dopaminergic D2 receptor in male germ cells. / Rodríguez-Gil, Joan Enric; Otth, Carola; Torres, Mauricio; Ramírez, Alfredo; Fernandez, Juan Carlos; Castro, Maite; Rauch, M. Cecilia; Brito, Mónica; Yañez, Alejandro J.; Slebe, Juan Carlos; Concha, Ilona I.

In: Journal of Cellular Biochemistry, Vol. 100, 01.01.2007, p. 141-150.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - Novel identification of peripheral dopaminergic D2 receptor in male germ cells

AU - Rodríguez-Gil, Joan Enric

AU - Otth, Carola

AU - Torres, Mauricio

AU - Ramírez, Alfredo

AU - Fernandez, Juan Carlos

AU - Castro, Maite

AU - Rauch, M. Cecilia

AU - Brito, Mónica

AU - Yañez, Alejandro J.

AU - Slebe, Juan Carlos

AU - Concha, Ilona I.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - Dopamine is a recognized modulator in the central nervous system (CNS) and peripheral organ functions. The presence of peripheral dopamine receptors outside the CNS has suggested an intriguing interaction between the nervous system and other functional systems, such as the reproductive system. In the present study we analyzed the expression of D2R receptors in rat testis, rat spermatogenic cells and spermatozoa, in different mammals. The RT-PCR analysis of rat testis mRNA showed specific bands corresponding to the two dopamine receptor D2R (L and S) isoforms previously described in the brain. Using Western blot analysis, we confirmed that the protein is present in rat testis, isolated spermatogenic cells and also in spermatozoa of a range of different mammals, such as rat, mouse, bull, and human. The immunohistochemistry analysis of rat adult testis showed that the receptor was expressed in all germ cells (pre- and post-meiotic phase) of the tubule with staining predominant in spermatogonia. Confocal analysis by indirect immunofluorescence revealed that in non-capacitated spermatozoa of rat, mouse, bull, and human, D2R is mainly localized in the flagellum, and is also observed in the acrosomal region of the sperm head (except in human spermatozoa). Our findings demonstrate that the two D2 receptor isoforms are expressed in rat testis and that the receptor protein is present in different mammalian spermatozoa. The presence of D2R receptors in male germ cells implies new and unsuspected roles for dopamine signaling in testicular and sperm physiology. © 2006 Wiley-Liss, Inc.

AB - Dopamine is a recognized modulator in the central nervous system (CNS) and peripheral organ functions. The presence of peripheral dopamine receptors outside the CNS has suggested an intriguing interaction between the nervous system and other functional systems, such as the reproductive system. In the present study we analyzed the expression of D2R receptors in rat testis, rat spermatogenic cells and spermatozoa, in different mammals. The RT-PCR analysis of rat testis mRNA showed specific bands corresponding to the two dopamine receptor D2R (L and S) isoforms previously described in the brain. Using Western blot analysis, we confirmed that the protein is present in rat testis, isolated spermatogenic cells and also in spermatozoa of a range of different mammals, such as rat, mouse, bull, and human. The immunohistochemistry analysis of rat adult testis showed that the receptor was expressed in all germ cells (pre- and post-meiotic phase) of the tubule with staining predominant in spermatogonia. Confocal analysis by indirect immunofluorescence revealed that in non-capacitated spermatozoa of rat, mouse, bull, and human, D2R is mainly localized in the flagellum, and is also observed in the acrosomal region of the sperm head (except in human spermatozoa). Our findings demonstrate that the two D2 receptor isoforms are expressed in rat testis and that the receptor protein is present in different mammalian spermatozoa. The presence of D2R receptors in male germ cells implies new and unsuspected roles for dopamine signaling in testicular and sperm physiology. © 2006 Wiley-Liss, Inc.

KW - D2 receptors

KW - Male germ cells

KW - Spermatozoa

U2 - https://doi.org/10.1002/jcb.21037

DO - https://doi.org/10.1002/jcb.21037

M3 - Article

VL - 100

SP - 141

EP - 150

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

ER -