Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Marta Jansà Pérez; Christine Leroux; Armand Sànchez Bonastre; Patrice Martin

doi:10.1016/S1050-3862(96)80015-5

Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Marta Jansà Pérez, Christine Leroux, Armand Sànchez Bonastre, Patrice Martin

Research output: Contribution to journal › Article › Research › peer-review

Abstract

Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

Original language	English
Pages (from-to)	143-145
Journal	Genetic analysis : biomolecular engineering
Volume	12
Issue number	3-4
DOIs	https://doi.org/10.1016/S1050-3862(96)80015-5
Publication status	Published - 1 Jan 1996

Keywords

Denaturing agents
Ethidium bromide staining
Goat α -casein E s1
Northern blotting

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10.1016/S1050-3862(96)80015-5

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title = "Northern analysis of highly folded goat αs1 Casein mRNA",

abstract = "Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.",

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AU - Leroux, Christine

AU - Sànchez Bonastre, Armand

AU - Martin, Patrice

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Y1 - 1996/1/1

N2 - Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

AB - Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.

KW - Denaturing agents

KW - Ethidium bromide staining

KW - Goat α -casein E s1

KW - Northern blotting

UR - https://www.scopus.com/pages/publications/0347934876

U2 - 10.1016/S1050-3862(96)80015-5

DO - 10.1016/S1050-3862(96)80015-5

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VL - 12

SP - 143

EP - 145

JO - Genetic analysis : biomolecular engineering

JF - Genetic analysis : biomolecular engineering

IS - 3-4

ER -

Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Abstract

Keywords

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