Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Marta Jansà Pérez, Christine Leroux, Armand Sànchez Bonastre, Patrice Martin

    Research output: Contribution to journalArticleResearchpeer-review


    Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.
    Original languageEnglish
    Pages (from-to)143-145
    JournalGenetic Analysis - Biomolecular Engineering
    Issue number3-4
    Publication statusPublished - 1 Jan 1996


    • Denaturing agents
    • Ethidium bromide staining
    • Goat α -casein E s1
    • Northern blotting


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