Northern analysis of highly folded goat α<inf>s1</inf> Casein mRNA

Marta Jansà Pérez, Christine Leroux, Armand Sànchez Bonastre, Patrice Martin

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.
    Original languageEnglish
    Pages (from-to)143-145
    JournalGenetic Analysis - Biomolecular Engineering
    Volume12
    Issue number3-4
    DOIs
    Publication statusPublished - 1 Jan 1996

    Keywords

    • Denaturing agents
    • Ethidium bromide staining
    • Goat α -casein E s1
    • Northern blotting

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