Northern blotting using glyoxal to denature a highly folded mRNA, such as goat αs1-Casein E, can lead to the detection of multiple incompletely denatured forms. Formaldehyde appears to be the most suitable agent for Northern blotting due to its effective denaturing capacity and lower toxicity than methylmercuric hydroxide.
|Journal||Genetic Analysis - Biomolecular Engineering|
|Publication status||Published - 1 Jan 1996|
- Denaturing agents
- Ethidium bromide staining
- Goat α -casein E s1
- Northern blotting