Non-random reconstitution of HMG1 and HMG2 in chromatin. Determination of the histone contacts

Jordi Bernués, Enrique Querol

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4 Citations (Scopus)

Abstract

We have studied how non-histone proteins HMG1 and HMG2 interact with rat liver chromatin using reconstitution and chemical cross-linking procedures. Both proteins were found to associate to chromatin only to some extent and always with a marked preference for short oligonucleosomes, mainly mono- and dinucleosomes. However, a slight reconstitution with the long polynucleosomal fraction can be observed in H1-depleted chromatin. Reconstitution is non-random and a clear preference for regions highly sensitive to staphylococcal nuclease (EC 3.1.31.1) is observed. Chemical cross-linking has allowed us to identify H1, H2A and H2B as the histones contacted by HMG1 and HMG2 upon reconstitution. Also, we present evidence that HMG2 and HMG2 interact with the nucleosomal particle without replacing H1 or any other histone. © 1989.
Original languageEnglish
Pages (from-to)52-61
JournalBiochimica et biophysica acta. General subjects
Volume0
Issue number1008
DOIs
Publication statusPublished - 1 Jun 1989

Keywords

  • (Calf thymus)
  • Chromatin-protein interaction
  • High mobility group protein
  • Nonhistone protein

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