Non-meiotic chromosome instability in human immature oocytes

Gemma Daina, Laia Ramos, Mariona Rius, Albert Obradors, Javier Del Rey, Magda Giralt, Mercedes Campillo, Esther Velilla, Aïda Pujol, Olga Martinez-Pasarell, Jordi Benet, Joaquima Navarro

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4 Citations (Scopus)

Abstract

Aneuploidy has been a major issue in human gametes and is closely related to fertility problems, as it is known to be present in cleavage stage embryos and gestational losses. Pre-meiotic chromosome abnormalities in women have been previously described. The aim of this study is to assess the whole-chromosome complement in immature oocytes to find those abnormalities caused by mitotic instability. For this purpose, a total of 157 oocytes at the germinal vesicle or metaphase I stage, and discarded from IVF cycles, were analysed by CGH. Fifty-six women, between 18 and 45 years old (mean 32.5 years), including 32 IVF patients (25-45 years of age) and 24 IVF oocyte donors (18-33 years of age), were included in the study. A total of 25/157 (15.9%) of the oocytes analysed, obtained from three IVF clinics, contained chromosome abnormalities, including both aneuploidy (24/157) and structural aberrations (9/157). Independently of the maternal age, the incidence of abnormal oocytes which originated before meiosis is 15.9%, and these imbalances were found in 33.9% of the females studied. This work sheds light on the relevance of mitotic instability responsible for the generation of the abnormalities present in human oocytes. © 2014 Macmillan Publishers Limited All rights reserved.
Original languageEnglish
Pages (from-to)202-207
JournalEuropean Journal of Human Genetics
Volume22
Issue number2
DOIs
Publication statusPublished - 1 Feb 2014

Keywords

  • aneuploidy
  • germline mitotic abnormalities
  • immature oocytes
  • premeiotic instability
  • segmental imbalances

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