Non-invasive grading of astrocytic tumours from the relative contents of myo-inositol and glycine measured by in vivo MRS

A. P. Candiota, C. Majós, M. Julià-Sapé, M. Cabañas, J. J. Acebes, A. Moreno-Torres, J. R. Griffiths, C. Arús

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19 Citations (Scopus)

Abstract

MRI and MRS are established methodologies for evaluating intracranial lesions. One MR spectral feature suggested for in vivo grading of astrocytic tumours is the apparent myo-Inositol (mI) intensity (ca 3.55ppm) at short echo times, although glycine (gly) may also contribute in vivo to this resonance. The purpose of this study was to quantitatively evaluate the mI + gly contribution to the recorded spectral pattern in vivo and correlate it with in vitro data obtained from perchloric acid extraction of tumour biopsies. Patient spectra (n = 95) at 1.5T at short (20-31 ms) and long (135-136 ms) echo times were obtained from the INTERPRET MRS database (http://gabrmn.uab.es/interpretvalidateddb/). Phantom spectra were acquired with a comparable protocol. Spectra were automatically processed and the ratios of the (mI + gly) to Cr peak heights ((mI + gly)/Cr) calculated. Perchloric acid extracts of brain tumour biopsies were analysed by high-resolution NMR at 9.4T. The ratio (mI + gly)/Cr decreased significantly with astrocytic grade in vivo between low-grade astrocytoma (A2) and glioblastoma multiforme (GBM). In vitro results displayed a somewhat different tendency, with anaplastic astrocytomas having significantly higher (mI + gly)/Cr than A2 and GBM. The discrepancy between in vivo and in vitro data suggests that the NMR visibility of glycine in glial brain tumours is restricted in vivo.
Original languageEnglish
Pages (from-to)319-329
JournalJBR-BTR
Volume94
Issue number6
DOIs
Publication statusPublished - 1 Jan 2011

Keywords

  • Astrocytoma - Magnetic resonance (MR)
  • spectroscopy

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