NMR studies of the conformation of thiocellobiose bound to a β-glucosidase from Streptomyces sp.

Esther Montero, Miquel Vallmitjana, Josep A. Pérez-Pons, Enrique Querol, Jesús Jiménez-Barbero, F. Javier Cañada

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Abstract

The conformation of 4-thiocellobiose bound to β-glucosidase from Streptromyces sp. has been studied by 1H-NMR transferred nuclear Overhauser effect spectroscopy (TR-NOE). Thiocellobiose behaves as an inhibitor of this glucosidase when cellobiose is used as substrate. NOE measurements and molecular mechanics calculations have also been performed to estimate the probability distribution of conformers of thiocellobiose when free in solution. Experimental data show that, in contrast with the natural O-analogue, thiocellobiose presents three conformational families in the free state, namely syn, anti-Ψ and anti-Φ whilst only one of them (syn) is recognized by the enzyme.
Original languageEnglish
Pages (from-to)243-248
JournalFEBS Letters
Volume421
DOIs
Publication statusPublished - 16 Jan 1998

Keywords

  • Molecular mchanics calculation
  • Streptomyces sp.
  • Thiocellobiose
  • Transferred NOE spectroscopy
  • β-glycosidase

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    Montero, E., Vallmitjana, M., Pérez-Pons, J. A., Querol, E., Jiménez-Barbero, J., & Cañada, F. J. (1998). NMR studies of the conformation of thiocellobiose bound to a β-glucosidase from Streptomyces sp. FEBS Letters, 421, 243-248. https://doi.org/10.1016/S0014-5793(97)01571-8