Nitric oxide as a putative non-adrenergic inhibitory transmiter in the canine pylorus in vivo

H D Allescher, G Tougas, P Vergara, S Lu, E E Daniel

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97 Citations (Scopus)


Antropyloroduodenal motility was recorded in seven anesthetized dogs to assess the role of nitric oxide and L-arginine metabolites in nonadrenergic noncholinergic (NANC) mediation of pyloric relaxation. Pyloric activity induced by duodenal field stimulation was inhibited by antral field stimulation and electrical vagal stimulation. Intra-arterial NG-L-arginine-methyl-ester (L-NAME) reduced the inhibition from antral or vagal stimulation (P less than 0.05). Intravenous infusion of L-NAME also blocked the inhibitory effect of vagal and antral stimulation but left the tetrodotoxin-insensitive action of intra-arterial vasoactive intestinal peptide (VIP) and sodium nitroprusside unchanged. L-Arginine reversed the effect of L-NAME whereas D-arginine did not. L-NAME enhanced pyloric contractions to intra-arterial acetylcholine. The NANC inhibition of the substance P-stimulated pyloric response in vitro was blocked by L-NAME and reversed by addition of L-arginine. Sodium nitroprusside was effective as a relaxant in vitro but VIP was not. These data suggest that metabolites of L-arginine mediate neural inhibition of canine pyloric motor activity.

Original languageEnglish
Pages (from-to)G695-702
JournalAmerican Journal of Physiology (Regulatory and Comparative Physiol.)
Issue number4 Pt 1
Publication statusPublished - Apr 1992


  • Animals
  • Arginine/analogs & derivatives
  • Dogs
  • Female
  • In Vitro Techniques
  • Male
  • Motor Activity/drug effects
  • Muscle, Smooth/innervation
  • NG-Nitroarginine Methyl Ester
  • Neural Inhibition
  • Neurotransmitter Agents/physiology
  • Nitric Oxide/metabolism
  • Nitroprusside/pharmacology
  • Pylorus/drug effects
  • Stereoisomerism
  • Vasoactive Intestinal Peptide/pharmacology


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