New steroidal 7-azaindole platinum(II) antitumor complexes

Ana Zamora, Venancio Rodríguez, Natalia Cutillas, Gorakh S. Yellol, Arturo Espinosa, Katia G. Samper, Mercè Capdevila, Òscar Palacios, José Ruiz

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18 Citations (Scopus)

Abstract

Two new steroidal 7-azaindole-based N-donor ligands 17-α-[7- azaindole-5-ethynyl]-17-β-testosterone (ET-Haza) (1) and 17-α-[7-azaindole-5-ethynyl]-19-nortestosterone (LEV-Haza) (2), and two new DNA damaging warheads with an enhanced lipophilicity [Pt(dmba)Cl(L)] (dmba = N,N-dimethylbenzylamine-κN,κC; L = ET-Haza (3) and LEV-Haza (4)) have been prepared and characterized. Values of IC50 were calculated for complexes 3 and 4 against a panel of human tumor cell lines representative of ovarian (A2780 and A2780cis) and breast cancers (T47D). At 48 h of incubation time 3 and 4 showed very low resistance factors (RF of 1) against an A2780 cell line which has acquired resistance to cisplatin, IC50 values of the new complexes towards normal human LLC-PK1 renal cells at 48 h being about double than that of cisplatin. 3 and 4 are able to react with 9-ethylguanine (9-EtG) yielding the corresponding monoadduct [Pt(dmba)(L)(9-EtG)]+ derivatives as followed by ESI-MS. Compound 3 interacts mainly with double-stranded (DS) oligonucleotides as shown by analysis with ESI-TOF-MS, being also able to displace ethidium bromide (EB) from DNA, as observed by an electrophoretic mobility study. 3 and 4 are good cathepsin B inhibitors. Theoretical calculations at the COSMO(CHCl3)/B3LYP-D/def2-TZVPPecp// B3LYP-D/def2-TZVPecp level and energy evaluations at the COSMO(CHCl 3)/PWPB95-D3/def2-TZVPPecp level of theory on compound 4 and model systems have been done. © 2013 Elsevier Inc.
Original languageEnglish
Pages (from-to)48-56
JournalJournal of Inorganic Biochemistry
Volume128
DOIs
Publication statusPublished - 8 Aug 2013

Keywords

  • Cancer cells
  • Cytotoxicity
  • DNA
  • Metal complex steroidal conjugate
  • Platinum complexes

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