New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance

Lorena Franco-Martínez; Diego Romero; Camila Peres Rubio; Fernando Tecles; Silvia Martínez-Subiela; Mariana Teles; Asta Tvarijonaviciute

doi:10.1016/j.cbpb.2018.04.006

New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance

Lorena Franco-Martínez, Diego Romero, Camila Peres Rubio, Fernando Tecles, Silvia Martínez-Subiela, Mariana Teles, Asta Tvarijonaviciute

Research output: Contribution to journal › Article › Research › peer-review

5 Citations (Scopus)

Abstract

© 2018 The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). The assays were precise, accurate and provided low limits of detection. When oxidative stress was promoted by inducing hypoxia and the behaviour of these biomarkers between hypoxic and controls mussels were compared, statistically significant differences were observed in all biomarkers and tissues evaluated. The results of the present study demonstrated that these biomarkers, not previously studied in mussels, show a potential use as biomarkers of oxidative stress in this species since they were validated and showed changes under a state of oxidative stress.

Original language	English
Pages (from-to)	44-49
Journal	Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume	221-222
DOIs	https://doi.org/10.1016/j.cbpb.2018.04.006
Publication status	Published - 1 Jul 2018

Keywords

Antioxidants
Assay validation
Hypoxia
Mussels
Novel biomarkers
Oxidants
Oxidative stress

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10.1016/j.cbpb.2018.04.006

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Franco-Martínez, L., Romero, D., Rubio, C. P., Tecles, F., Martínez-Subiela, S., Teles, M., & Tvarijonaviciute, A. (2018). New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance. Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, 221-222, 44-49. https://doi.org/10.1016/j.cbpb.2018.04.006

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title = "New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance",

abstract = "{\textcopyright} 2018 The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). The assays were precise, accurate and provided low limits of detection. When oxidative stress was promoted by inducing hypoxia and the behaviour of these biomarkers between hypoxic and controls mussels were compared, statistically significant differences were observed in all biomarkers and tissues evaluated. The results of the present study demonstrated that these biomarkers, not previously studied in mussels, show a potential use as biomarkers of oxidative stress in this species since they were validated and showed changes under a state of oxidative stress.",

keywords = "Antioxidants, Assay validation, Hypoxia, Mussels, Novel biomarkers, Oxidants, Oxidative stress",

author = "Lorena Franco-Mart{\'i}nez and Diego Romero and Rubio, {Camila Peres} and Fernando Tecles and Silvia Mart{\'i}nez-Subiela and Mariana Teles and Asta Tvarijonaviciute",

year = "2018",

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doi = "10.1016/j.cbpb.2018.04.006",

language = "English",

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pages = "44--49",

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Franco-Martínez, L, Romero, D, Rubio, CP, Tecles, F, Martínez-Subiela, S, Teles, M & Tvarijonaviciute, A 2018, 'New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance', Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, vol. 221-222, pp. 44-49. https://doi.org/10.1016/j.cbpb.2018.04.006

New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance. / Franco-Martínez, Lorena; Romero, Diego; Rubio, Camila Peres et al.

In: Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology, Vol. 221-222, 01.07.2018, p. 44-49.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance

AU - Franco-Martínez, Lorena

AU - Romero, Diego

AU - Rubio, Camila Peres

AU - Tecles, Fernando

AU - Martínez-Subiela, Silvia

AU - Teles, Mariana

AU - Tvarijonaviciute, Asta

PY - 2018/7/1

Y1 - 2018/7/1

N2 - © 2018 The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). The assays were precise, accurate and provided low limits of detection. When oxidative stress was promoted by inducing hypoxia and the behaviour of these biomarkers between hypoxic and controls mussels were compared, statistically significant differences were observed in all biomarkers and tissues evaluated. The results of the present study demonstrated that these biomarkers, not previously studied in mussels, show a potential use as biomarkers of oxidative stress in this species since they were validated and showed changes under a state of oxidative stress.

AB - © 2018 The aim of the present report was to develop and validate new automated spectrophotometric assays for measurement of total antioxidant capacity (TAC), thiols, and advanced oxidation protein products (AOPP) in mussel gills, digestive gland and hemolymph samples, and to evaluate their possible utility in biomonitoring programs. Total antioxidant capacity (TAC) was measured by different methods: trolox equivalent antioxidant capacity (TEAC1 and TEAC2), cupric reducing antioxidant capacity (CUPRAC), and ferric reducing ability of plasma (FRAP). The assays were precise, accurate and provided low limits of detection. When oxidative stress was promoted by inducing hypoxia and the behaviour of these biomarkers between hypoxic and controls mussels were compared, statistically significant differences were observed in all biomarkers and tissues evaluated. The results of the present study demonstrated that these biomarkers, not previously studied in mussels, show a potential use as biomarkers of oxidative stress in this species since they were validated and showed changes under a state of oxidative stress.

KW - Antioxidants

KW - Assay validation

KW - Hypoxia

KW - Mussels

KW - Novel biomarkers

KW - Oxidants

KW - Oxidative stress

U2 - 10.1016/j.cbpb.2018.04.006

DO - 10.1016/j.cbpb.2018.04.006

M3 - Article

VL - 221-222

SP - 44

EP - 49

ER -

New potential biomarkers of oxidative stress in Mytilus galloprovincialis: Analytical validation and overlap performance

Abstract

Keywords

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