New PCR method to differentiate species in the Aspergillus niger aggregate

F. Accensi; J. Cano; L. Figuera; M. L. Abarca; F. J. Cabañes

doi:10.1016/S0378-1097(99)00485-1

New PCR method to differentiate species in the Aspergillus niger aggregate

F. Accensi, J. Cano, L. Figuera, M. L. Abarca, F. J. Cabañes

Research output: Contribution to journal › Article › Research › peer-review

105 Citations (Scopus)

Abstract

The DNA that encodes the 5.8S gene of the ribosomal RNA and the two intergenic spacers ITS1 and ITS2 of the two proposed type strains of the Aspergillus niger aggregate (A. niger and Aspergillus tubingensis) have been sequenced. By comparison of sequences we have found that both species could be differentiated by RsaI digestion of the PCR products of the mentioned regions. This method could be a useful tool in the identification of strains of the A. niger aggregate, especially in studies that involve a large number of isolates. Copyright (C) 1999 Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	191-196
Journal	FEMS Microbiology Letters
Volume	180
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(99)00485-1
Publication status	Published - 15 Nov 1999

Keywords

5.8S rDNA
Aspergillus niger
Aspergillus tubingensis
PCR
RFLP analysis
Taxonomy

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10.1016/S0378-1097(99)00485-1

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title = "New PCR method to differentiate species in the Aspergillus niger aggregate",

abstract = "The DNA that encodes the 5.8S gene of the ribosomal RNA and the two intergenic spacers ITS1 and ITS2 of the two proposed type strains of the Aspergillus niger aggregate (A. niger and Aspergillus tubingensis) have been sequenced. By comparison of sequences we have found that both species could be differentiated by RsaI digestion of the PCR products of the mentioned regions. This method could be a useful tool in the identification of strains of the A. niger aggregate, especially in studies that involve a large number of isolates. Copyright (C) 1999 Federation of European Microbiological Societies.",

keywords = "5.8S rDNA, Aspergillus niger, Aspergillus tubingensis, PCR, RFLP analysis, Taxonomy",

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AU - Accensi, F.

AU - Cano, J.

AU - Figuera, L.

AU - Abarca, M. L.

AU - Cabañes, F. J.

PY - 1999/11/15

Y1 - 1999/11/15

N2 - The DNA that encodes the 5.8S gene of the ribosomal RNA and the two intergenic spacers ITS1 and ITS2 of the two proposed type strains of the Aspergillus niger aggregate (A. niger and Aspergillus tubingensis) have been sequenced. By comparison of sequences we have found that both species could be differentiated by RsaI digestion of the PCR products of the mentioned regions. This method could be a useful tool in the identification of strains of the A. niger aggregate, especially in studies that involve a large number of isolates. Copyright (C) 1999 Federation of European Microbiological Societies.

AB - The DNA that encodes the 5.8S gene of the ribosomal RNA and the two intergenic spacers ITS1 and ITS2 of the two proposed type strains of the Aspergillus niger aggregate (A. niger and Aspergillus tubingensis) have been sequenced. By comparison of sequences we have found that both species could be differentiated by RsaI digestion of the PCR products of the mentioned regions. This method could be a useful tool in the identification of strains of the A. niger aggregate, especially in studies that involve a large number of isolates. Copyright (C) 1999 Federation of European Microbiological Societies.

KW - 5.8S rDNA

KW - Aspergillus niger

KW - Aspergillus tubingensis

KW - PCR

KW - RFLP analysis

KW - Taxonomy

U2 - 10.1016/S0378-1097(99)00485-1

DO - 10.1016/S0378-1097(99)00485-1

M3 - Article

VL - 180

SP - 191

EP - 196

IS - 2

ER -

New PCR method to differentiate species in the Aspergillus niger aggregate

Abstract

Keywords

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