New antibodies immobilization system into a graphite-polysulfone membrane for amperometric immunosensors

Samuel Sánchez Ordóñez, Esteve Fàbregas

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

Polysulfone membrane is used for the first time for the preparation of electrochemical immunosensors. A disposable immunosensor based on a porous conductor polymer graphite-polysulfone-electrode has been developed using a phase inversion technique for the determination of anti-rabbit IgG (anti-RIgG) as a model analyte. To construct the sensor, a conductor membrane was deposited on the surface of working graphite-epoxy composite (GEC) electrode. The membrane was characterized by SEM. This sensor was based on the competitive assay between free and labeled anti-RIgG for the available binding sites of immobilized rabbit IgG (RIgG). Incubation parameters were optimized in this work. The immunological reaction was detected using an enzymatic-labeling procedure (HRP enzyme) combined with the amperometric detection using H2O2 as substrate and hydroquinone as mediator. This sensor shows stability during a week and a good reproducibility. The current was monitored amperometrically at -0.1 V versus SCE and this method showed a linear range of the anti-RIgG from 1 to 6 μg/ml. The detection limit was determined to be 0.77 μg/ml. © 2006 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)965-972
JournalBiosensors and Bioelectronics
Volume22
Issue number6
DOIs
Publication statusPublished - 15 Jan 2007

Keywords

  • Amperometric biosensor
  • Antibody immobilization
  • Biocomposite
  • Composite
  • Immunosensor
  • Polysulfone membrane

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