Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens

Aida J. Chaves, Núria Busquets, Rosa Valle, Raquel Rivas, Júlia Vergara-Alert, Roser Dolz, Antonio Ramis, Ayub Darji, Natlia Majá

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)


In order to understand the mechanism of neuroinvasion of a highly pathogenic avian influenza virus (HPAIV) into the central nervous system (CNS) of chickens, specific pathogen free chickens were inoculated with a H7N1 HPAIV. Blood, cerebrospinal fluid (CSF), nasal cavity and brain tissue samples were obtained from 1 to 4 days post-inoculation (dpi) of infected and control chickens. Viral antigen topographical distribution, presence of influenza A virus receptors in the brain, as well as, the role of the olfactory route in virus CNS invasion were studied using different immunohistochemistry techniques. Besides, viral RNA load in CSF and blood was quantified by means of a quantitative real-time reverse transcription-polymerase chain reaction. Viral antigen was observed widely distributed in the CNS, showing bilateral and symmetrical distribution in the nuclei of the diencephalon, mesencephalon and rhombencephalon. Viral RNA was detected in blood and CSF at one dpi, indicating that the virus crosses the blood-CSF-barrier early during infection. This early dissemination is possibly favoured by the presence of Sia2,3 Gal and Sia2,6 Gal receptors in brain vascular endothelial cells, and Sia2,3 Gal receptors in ependymal and choroid plexus cells. No viral antigen was observed in olfactory sensory neurons, while the olfactory bulb showed only weak staining, suggesting that the virus did not use this pathway to enter into the brain. The sequence of virus appearance and the topographical distribution of this H7N1 HPAIV indicate that the viral entry occurs via the haematogenous route, with early and generalized spreading through the CSF. © 2011 Chaves et al; licensee BioMed Central Ltd.
Original languageEnglish
Article number106
JournalVeterinary Research
Publication statusPublished - 1 Dec 2011

Fingerprint Dive into the research topics of 'Neuropathogenesis of a highly pathogenic avian influenza virus (H7N1) in experimentally infected chickens'. Together they form a unique fingerprint.

Cite this