Nanoliposomes encapsulating immunostimulants modulate the innate immune system and elicit protection in zebrafish larvae

Jie Ji, Susana Merino, Juan M. Tomás, Nerea Roher

Research output: Contribution to journalArticleResearch

7 Citations (Scopus)

Abstract

© 2019 Elsevier Ltd Here we present immunostimulant-loaded nanoliposomes (NLc) as a strategy to protect zebrafish larvae against bacterial infection. The NLc encapsulate crude lipopolysaccharide (LPS) from E. coli and polyinosinic:polycytidylic acid (Poly I:C), a synthetic analogue of viral dsRNA. Fluorescently-labeled NLc were ingested by zebrafish larvae 4 days post fertilization, when administrated by bath immersion, and accumulated in the intestine. RT-qPCR analysis showed the expression of innate immune related genes (tnfα, il1β, nos2a, irf1a and ptgs2a) was significantly upregulated at 48 h post NLc treatment. A zebrafish larvae infection model for Aeromonas hydrophila was set up by bath immersion, achieving bacterial-dose-dependent significant differences in survival at day 5 post infection in both injured and non-injured larvae. Using this model, NLc protected non-injured zebrafish larvae against an A. hydrophila lethal infection. In contrast, neither the empty nanoliposomes nor the mixture of immunostimulants could protect larvae against lethal challenges. Our results demonstrate that nanoliposomes could be further developed as an efficient carrier, widening the scope for delivery of other immunostimulants in aquaculture.
Original languageEnglish
Pages (from-to)421-429
JournalFish and Shellfish Immunology
Volume92
DOIs
Publication statusPublished - 1 Sep 2019

Keywords

  • Aeromonas hydrophila
  • Immune response
  • Nanoliposome
  • Zebrafish larvae

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