NAADP mediates ATP-induced Ca2+ signals in astrocytes

Miquel Barceló-Torns; Alexander M. Lewis; Albert Gubern; David Barneda; Duncan Bloor-Young; Fernado Picatoste; Grant C. Churchill; Enrique Claro; Roser Masgrau

doi:https://doi.org/10.1016/j.febslet.2011.05.062

NAADP mediates ATP-induced Ca²⁺ signals in astrocytes

Miquel Barceló-Torns, Alexander M. Lewis, Albert Gubern, David Barneda, Duncan Bloor-Young, Fernado Picatoste, Grant C. Churchill, Enrique Claro, Roser Masgrau^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Research › peer-review

22 Citations (Scopus)

Abstract

Intracellular Ca²⁺ signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca²⁺ in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca²⁺ responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes.

Original language	English
Pages (from-to)	2300-2306
Number of pages	7
Journal	Febs letters
Volume	585
Issue number	14
DOIs	https://doi.org/10.1016/j.febslet.2011.05.062
Publication status	Published - 21 Jul 2011

Keywords

Bradykinin
Ca mobilization
Endotehlin-1
Glia
Lysosome
Ned-19

Access to Document

https://doi.org/10.1016/j.febslet.2011.05.062

https://ddd.uab.cat/record/170687

Cite this

@article{e06cd2a50bf142c584f112b34fcc498c,

title = "NAADP mediates ATP-induced Ca2+ signals in astrocytes",

abstract = "Intracellular Ca2+ signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca2+ in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca2+ responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes.",

keywords = "Bradykinin, Ca mobilization, Endotehlin-1, Glia, Lysosome, Ned-19",

author = "Miquel Barcel{\'o}-Torns and Lewis, {Alexander M.} and Albert Gubern and David Barneda and Duncan Bloor-Young and Fernado Picatoste and Churchill, {Grant C.} and Enrique Claro and Roser Masgrau",

note = "Funding Information: We would like to thank Dr. Anthony Morgan for his help with Confocal Microscopy. This research was funded by grants PI05/1723 from the Spanish Ministry of Health (Instituto Carlos III) and E.U. grant MERG-6-CT-2005-014855. M.B.-T. was recipient of a fellowship from Ministerio de Educaci{\'o}n y Ciencia. A.G. and D.B. were recipients of a fellowship from UAB and Generalitat de Catalunya, respectively. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

year = "2011",

month = jul,

day = "21",

doi = "https://doi.org/10.1016/j.febslet.2011.05.062",

language = "English",

volume = "585",

pages = "2300--2306",

number = "14",

}

TY - JOUR

T1 - NAADP mediates ATP-induced Ca2+ signals in astrocytes

AU - Barceló-Torns, Miquel

AU - Lewis, Alexander M.

AU - Gubern, Albert

AU - Barneda, David

AU - Bloor-Young, Duncan

AU - Picatoste, Fernado

AU - Churchill, Grant C.

AU - Claro, Enrique

AU - Masgrau, Roser

N1 - Funding Information: We would like to thank Dr. Anthony Morgan for his help with Confocal Microscopy. This research was funded by grants PI05/1723 from the Spanish Ministry of Health (Instituto Carlos III) and E.U. grant MERG-6-CT-2005-014855. M.B.-T. was recipient of a fellowship from Ministerio de Educación y Ciencia. A.G. and D.B. were recipients of a fellowship from UAB and Generalitat de Catalunya, respectively. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2011/7/21

Y1 - 2011/7/21

N2 - Intracellular Ca2+ signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca2+ in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca2+ responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes.

AB - Intracellular Ca2+ signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca2+ in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca2+ responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes.

KW - Bradykinin

KW - Ca mobilization

KW - Endotehlin-1

KW - Glia

KW - Lysosome

KW - Ned-19

UR - http://www.scopus.com/inward/record.url?scp=79960364987&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.febslet.2011.05.062

DO - https://doi.org/10.1016/j.febslet.2011.05.062

M3 - Article

C2 - 21664355

AN - SCOPUS:79960364987

VL - 585

SP - 2300

EP - 2306

IS - 14

ER -

NAADP mediates ATP-induced Ca²⁺ signals in astrocytes

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this