NAADP mediates ATP-induced Ca2+ signals in astrocytes

Miquel Barceló-Torns, Alexander M. Lewis, Albert Gubern, David Barneda, Duncan Bloor-Young, Fernado Picatoste, Grant C. Churchill, Enrique Claro, Roser Masgrau

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

Intracellular Ca2+ signals provide astrocytes with a specific form of excitability that enables them to regulate synaptic transmission. In this study, we demonstrate that NAADP-AM, a membrane-permeant analogue of the new second messenger nicotinic acid-adenine dinucleotide phosphate (NAADP), mobilizes Ca2+ in astrocytes and that the response is blocked by Ned-19, an antagonist of NAADP signalling. We also show that NAADP receptors are expressed in lysosome-related acidic vesicles. Pharmacological disruption of either NAADP or lysosomal signalling reduced Ca2+ responses induced by ATP and endothelin-1, but not by bradykinin. Furthermore, ATP increased endogenous NAADP levels. Overall, our data provide evidence for NAADP being an intracellular messenger for agonist-mediated calcium signalling in astrocytes. © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)2300-2306
JournalFEBS Letters
Volume585
DOIs
Publication statusPublished - 21 Jul 2011

Keywords

  • Bradykinin
  • Ca mobilization 2+
  • Endotehlin-1
  • Glia
  • Lysosome
  • Ned-19

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