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Abstract
Intravesical Mycobacterium bovis Bacillus Calmette-Guérin (BCG) immunotherapy remains the gold-standard treatment for non-muscle-invasive bladder cancer patients, even though half of the patients develop adverse events to this therapy. On exploring BCG-alternative therapies, Mycolicibacterium brumae, a nontuberculous mycobacterium, has shown outstanding anti-tumor and immunomodulatory capabilities. As no infections due to M. brumae in humans, animals, or plants have been described, the safety and/or toxicity of this mycobacterium have not been previously addressed. In the present study, an analysis was made of M. brumae- and BCG-intravenously-infected severe combined immunodeficient (SCID) mice, M. brumae-intravesically-treated BALB/c mice, and intrahemacoelic-infected-Galleria mellonella larvae. Organs from infected mice and the hemolymph from larvae were processed to count bacterial burden. Blood samples from mice were also taken, and a wide range of hematological and biochemical parameters were analyzed. Finally, histopathological alterations in mouse tissues were evaluated. Our results demonstrate the safety and non-toxic profile of M. brumae. Differences were observed in the biochemical, hematological and histopathological analysis between M. brumae and BCG-infected mice, as well as survival curves rates and colony forming units (CFU) counts in both animal models. M. brumae constitutes a safe therapeutic biological agent, overcoming the safety and toxicity disadvantages presented by BCG in both mice and G. mellonella animal models.
Original language | American English |
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Article number | 198 |
Journal | Vaccines |
Volume | 8 |
Issue number | 2 |
DOIs | |
Publication status | Published - 25 Apr 2020 |
Keywords
- BCG
- Bladder cancer
- Galleria mellonella
- Mice
- Nontuberculous mycobacteria
- Safety
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- 1 Finished
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DESCIFRANDO LAS INTERACCIONES MICOBACTERIA-HUESPED ESENCIALES PARA SU USO EN EL TRATAMIENTO DEL CANCER
Julian Gomez, E. (Principal Investigator), Luquin Fernandez, M. D. L. M. (Investigator) & Rabanal Prados, R. M. (Investigator)
Spanish Ministry of Economy and Competitiveness (MINECO)
1/01/19 → 30/09/22
Project: Research Projects and Other Grants