Mycobacterium detection method combining filtration, immunomagnetic separation, and electrochemical readout in a portable biosensing device

Melania Mesas Gómez, Bárbara Molina-Moya, Bárbara Camila de Araújo, Arnau Pallarès-Rusiñol, Jofre Ferrer-Dalmau, Maria Valnice Boldrín Zanoni, José Domínguez, Esther Julian, María Isabel Pividori*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)
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Abstract

This study addresses a straightforward and highly sensitive approach for detecting Mycobacterium fortuitum. The method involves a combination of filtration and direct immunomagnetic separation to isolate the bacteria retained on filters for further electrochemical magneto immunosensing in a handheld device. Unlike conventional methods involving pre-enrichment by culturing, this approach employs a simplified preconcentration technique, which includes filtration of large water samples, up to 100 mL, followed by a one-step process of immunomagnetic separation and labeling. After a 60-min, during which the filter with the retained bacteria and all the reagents (including modified magnetic particles and enzymatic conjugates) are incubated, the resulting product is directly drop into a cartridge, capable of performing magnetic actuation and washing. The electrochemical readout is carried out on a portable battery-operated device within 30 seconds. Remarkably, the immunosensor demonstrates an outstanding limit of detection of 5 CFU mL−1 in hemodialysis water processing 100 mL of sample. This achievement is remarkable considering the short and simplified analytical procedure, compared to the traditional isolation and culturing of mycobacteria, which typically takes 2 weeks.

Original languageEnglish
Article number135211
Pages (from-to)135211
Number of pages8
JournalSensors and Actuators B: Chemical
Volume403
DOIs
Publication statusPublished - 15 Mar 2024

Keywords

  • Handheld device
  • Hemodialysis water
  • Immunomagnetic separation
  • Mycobacterium fortuitum
  • PEM
  • Preconcentration strategy

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