Multiplexed detection of foodborne pathogens based on magnetic particles

Delfina Brandão, Susana Liébana, María Isabel Pividori

Research output: Contribution to journalArticleResearchpeer-review

44 Citations (Scopus)

Abstract

© 2015 Elsevier B.V. This paper addresses the novel approaches for the multiplex detection of food poisoning bacteria, paying closer attention to three of the most common pathogens involved in food outbreaks: Salmonella enterica, Escherichia coli O157:H7 and Listeria monocytogenes. End-point and real-time PCR, classical immunological techniques, biosensors, microarrays and microfluidic platforms, as well as commercial kits for multiplex detection of food pathogens will be reviewed, with special focus on the role of magnetic particles in these approaches. Although the immunomagnetic separation for capturing single bacteria from contaminating microflora and interfering food components has demonstrated to improve the performance on these approaches, the integration of magnetic particles for multiplex detection of bacteria is still in a preliminary stage and requires further studies.
Original languageEnglish
Pages (from-to)511-520
JournalNew Biotechnology
Volume32
Issue number5
DOIs
Publication statusPublished - 5 Sep 2015

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