Multiple sclerosis candidate autoantigens except myelin oligodendrocyte glycoprotein are transcribed in human thymus

Roxana Bruno, Lidia Sabater, Mireia Sospedra, Xavier Ferrer-Francesch, Domingo Escudero, Eva Martínez-Cáceres, Ricardo Pujol-Borrell

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72 Citations (Scopus)

Abstract

An important feature of central nervous system (CNS) immune privilege is that antigens expressed in CNS are sequestered and not available for central tolerance induction. Tissue distribution and, more specifically, thymic expression of many of the CNS putative autoantigens have not yet been clearly established in humans. We have addressed this question for the putative multiple sclerosis (MS) autoantigens αB-crystallin, S100β, proteolipid protein (PLP) and myelin oligodendrocyte glycoprotein (MOG)-α and MOG-β isoforms, using quantitative RT-PCR on human thymus (total, cell fractions and microdissected specimen) and on a panel of peripheral tissues. αB-crystallin, S100β and the DM20 isoform of PLP were clearly expressed in the thymus and also in selected peripheral tissues. In contrast, the expression of MOG out of the CNS was not observed. Within the human thymus, the level of CNS antigen expression was found higher in the stromal epithelial enriched cell fraction, and in micro-dissected samples of the medullary compartment. These results indicate that most of the antigens involved in MS are expressed in the thymus, suggesting a possible role in central tolerance. However, MOG and, to a lesser extent PLP, conform the classical concept of sequestered antigens, thus supporting the involvement of MOG in autoimmune demyelinating diseases.
Original languageEnglish
Pages (from-to)2737-2747
JournalEuropean Journal of Immunology
Volume32
Issue number10
DOIs
Publication statusPublished - 1 Oct 2002

Keywords

  • Multiple sclerosis
  • Myelin oligodendrocyte glycoprotein
  • Thymus
  • Tolerance

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