Multiple oncogenic mutations and clonal relationship in spatially distinct benign human epidermal tumors

Christian Hafner, Agustí Toll, Alejandro Fernández-Casado, Julie Earl, Miriam Marqués, Francesco Acquadro, Marinela Méndez-Pertuz, Miguel Urioste, Núria Malats, Julie E. Burns, Margaret A. Knowles, Juan C. Cigudosa, Arndt Hartmann, Thomas Vogt, Michael Landthaler, Ramón M. Pujol, Francisco X. Real

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64 Citations (Scopus)

Abstract

Malignant tumors result from the accumulation of genetic alterations in oncogenes and tumor suppressor genes. Much less is known about the genetic changes in benign tumors. Seborrheic keratoses (SK) are very frequent benign human epidermal tumors without malignant potential. We performed a comprehensive mutational screen of genes in the FGFR3-RAS-MAPK and phosphoinositide 3-kinase (PI3K)-AKT pathways from 175 SK, including multiple lesions from each patient. SK commonly harbored multiple bona fide oncogenic mutations in FGFR3, PIK3CA, KRAS, HRAS, EGFR, and AKT1 oncogenes but not in tumor suppressor genes TSC1 and PTEN. Despite the occurrence of oncogenic mutations and the evidence for downstream ERK/MAPK and PI3K pathway signaling, we did not find induction of senescence or a DNA damage response. Array comparative genomic hybridization (aCGH) analysis revealed that SK are genetically stable. The pattern of oncogenic mutations and X chromosome inactivation departs significantly from randomness and indicates that spatially independent lesions from a given patient share a clonal relationship. Our findings show that multiple oncogenic mutations in the major signaling pathways involved in cancer are not sufficient to drive malignant tumor progression. Furthermore, our data provide clues on the origin and spread of oncogenic mutations in tissues, suggesting that apparently independent (multicentric) adult benign tumors may have a clonal origin.
Original languageEnglish
Pages (from-to)20780-20785
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number48
DOIs
Publication statusPublished - 30 Nov 2010

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    Hafner, C., Toll, A., Fernández-Casado, A., Earl, J., Marqués, M., Acquadro, F., Méndez-Pertuz, M., Urioste, M., Malats, N., Burns, J. E., Knowles, M. A., Cigudosa, J. C., Hartmann, A., Vogt, T., Landthaler, M., Pujol, R. M., & Real, F. X. (2010). Multiple oncogenic mutations and clonal relationship in spatially distinct benign human epidermal tumors. Proceedings of the National Academy of Sciences of the United States of America, 107(48), 20780-20785. https://doi.org/10.1073/pnas.1008365107