Multicentric evaluation of the homogeneous LDL-cholesterol Plus assay: Comparison with beta-quantification and Friedewald formula

Margarita Esteban-Salan, José Angel Aguilar-Doreste, Maria Luisa Arranz-Pena, Santiago Juve-Cuxart, Ignasi Gich-Salarich, Edgar Zapico-Muniz, Jordi Ordonez-Llanos

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11 Citations (Scopus)

Abstract

Objectives: To evaluate the analytical and clinical performance of a new version of the LDL-C Plus assay and compare it with the beta-quantification (BQ) method in a multicenter study. Design and methods: Direct LDL-C was measured in 169 fresh pooled samples and in 830 clinical samples with known LDL-C by BQ. The reactivity of lipoproteins and the effect of hemoglobin, bilirubin and chylomicrons (CM) were studied. Results: Direct LDL-C total imprecision was < 2.2%; inaccuracy < ± 2.5% (unaffected by triglycerides up to 9.5 mmol/L); and total error 9.8%. Direct assay reacted with 95%, 50% and 25% of the cholesterol in the LDL, intermediate (IDL) and VLDL fractions, respectively. A significant association was observed with BQ. Icteric samples showed a negative bias and the effect of CM was variable. A positive bias was observed when VLDL-cholesterol/triglyceride ratio was > 0.57. Conclusions: LDL-C Plus assay represents a valid alternative to BQ for clinical laboratories. © 2008 The Canadian Society of Clinical Chemists.
Original languageEnglish
Pages (from-to)1402-1409
JournalClinical Biochemistry
Volume41
Issue number16-17
DOIs
Publication statusPublished - 1 Nov 2008

Keywords

  • Direct method
  • LDL-cholesterol
  • Method comparison

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