Multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from Spanish patients

Guillermo Quindós; Lourdes Abarca; Alfonso J. Carrillo-Muñoz; Ma Pilar Arévalo; Fernando J. Bornay; José B. Casals; Juan Manuel Hernández Molina; Isabel Iglesias; Ma José Linares; Estrella Martín-Mazuelos; Manuel Pereiro Ferreirós; Antonio Rezusta; Ma Carmen Rubio; Ricardo Salesa; Rosario San Millán; Josep M. Torres-Rodríguez

Multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from Spanish patients

Guillermo Quindós, Lourdes Abarca, Alfonso J. Carrillo-Muñoz, Ma Pilar Arévalo, Fernando J. Bornay, José B. Casals, Juan Manuel Hernández Molina, Isabel Iglesias, Ma José Linares, Estrella Martín-Mazuelos, Manuel Pereiro Ferreirós, Antonio Rezusta, Ma Carmen Rubio, Ricardo Salesa, Rosario San Millán, Josep M. Torres-Rodríguez

Department of Genetics and Microbiology

Research output: Contribution to journal › Article › Research › peer-review

17 Citations (Scopus)

Abstract

Twelve Spanish laboratories collected 325 yeast clinical isolates during a 30 day's period, among them 224 Candida albicans, 30 Candida glabrata, and 27 Candida parapsilosis. In vitro antifungal susceptibility to amphotericin B, ketoconazole, fluconazole and itraconazole was determined by an agar diffusion test (Neo-Sensitabs®, Rosco, Denmark). All the isolates tested were susceptible in vitro to amphotericin B and nearly all (97.2%) to itraconazole. In vitro susceptibility to fluconazole and ketoconazole was high (90.2% and 91.4% of isolates, respectively) but showed variations depending on the species tested. Resistance to fluconazole and ketoconazole was low in C. albicans (4% and 3%, respectively), but 30% of Candida guilliermondii and 36% of C. glabrata isolates were resistant to fluconazole. Ketoconazole resistance was observed in 40% of C. glabrata, and 17% of Candida tropicalis. Resistance to antifungal drugs is very low in Spain and it is related to non-C. albicans isolates.

Original language	English
Pages (from-to)	97-100
Journal	Revista Iberoamericana de Micologia
Volume	16
Issue number	2
Publication status	Published - 1 Jun 1999

Keywords

Agar diffusion
Antifungal susceptibility
Candida
Cross-resistance
Multicenter
Spain
Yeasts

Cite this

Quindós, G., Abarca, L., Carrillo-Muñoz, A. J., Arévalo, M. P., Bornay, F. J., Casals, J. B., Hernández Molina, J. M., Iglesias, I., Linares, M. J., Martín-Mazuelos, E., Ferreirós, M. P., Rezusta, A., Rubio, M. C., Salesa, R., Millán, R. S., & Torres-Rodríguez, J. M. (1999). Multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from Spanish patients. Revista Iberoamericana de Micologia, 16(2), 97-100.

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title = "Multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from Spanish patients",

abstract = "Twelve Spanish laboratories collected 325 yeast clinical isolates during a 30 day's period, among them 224 Candida albicans, 30 Candida glabrata, and 27 Candida parapsilosis. In vitro antifungal susceptibility to amphotericin B, ketoconazole, fluconazole and itraconazole was determined by an agar diffusion test (Neo-Sensitabs{\textregistered}, Rosco, Denmark). All the isolates tested were susceptible in vitro to amphotericin B and nearly all (97.2%) to itraconazole. In vitro susceptibility to fluconazole and ketoconazole was high (90.2% and 91.4% of isolates, respectively) but showed variations depending on the species tested. Resistance to fluconazole and ketoconazole was low in C. albicans (4% and 3%, respectively), but 30% of Candida guilliermondii and 36% of C. glabrata isolates were resistant to fluconazole. Ketoconazole resistance was observed in 40% of C. glabrata, and 17% of Candida tropicalis. Resistance to antifungal drugs is very low in Spain and it is related to non-C. albicans isolates.",

keywords = "Agar diffusion, Antifungal susceptibility, Candida, Cross-resistance, Multicenter, Spain, Yeasts",

author = "Guillermo Quind{\'o}s and Lourdes Abarca and Carrillo-Mu{\~n}oz, {Alfonso J.} and Ar{\'e}valo, {Ma Pilar} and Bornay, {Fernando J.} and Casals, {Jos{\'e} B.} and {Hern{\'a}ndez Molina}, {Juan Manuel} and Isabel Iglesias and Linares, {Ma Jos{\'e}} and Estrella Mart{\'i}n-Mazuelos and Ferreir{\'o}s, {Manuel Pereiro} and Antonio Rezusta and Rubio, {Ma Carmen} and Ricardo Salesa and Mill{\'a}n, {Rosario San} and Torres-Rodr{\'i}guez, {Josep M.}",

year = "1999",

month = jun,

day = "1",

language = "English",

volume = "16",

pages = "97--100",

journal = "Revista Iberoamericana de Micologia",

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Quindós, G, Abarca, L, Carrillo-Muñoz, AJ, Arévalo, MP, Bornay, FJ, Casals, JB, Hernández Molina, JM, Iglesias, I, Linares, MJ, Martín-Mazuelos, E, Ferreirós, MP, Rezusta, A, Rubio, MC, Salesa, R, Millán, RS & Torres-Rodríguez, JM 1999, 'Multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from Spanish patients', Revista Iberoamericana de Micologia, vol. 16, no. 2, pp. 97-100.

TY - JOUR

T1 - Multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from Spanish patients

AU - Quindós, Guillermo

AU - Abarca, Lourdes

AU - Carrillo-Muñoz, Alfonso J.

AU - Arévalo, Ma Pilar

AU - Bornay, Fernando J.

AU - Casals, José B.

AU - Hernández Molina, Juan Manuel

AU - Iglesias, Isabel

AU - Linares, Ma José

AU - Martín-Mazuelos, Estrella

AU - Ferreirós, Manuel Pereiro

AU - Rezusta, Antonio

AU - Rubio, Ma Carmen

AU - Salesa, Ricardo

AU - Millán, Rosario San

AU - Torres-Rodríguez, Josep M.

PY - 1999/6/1

Y1 - 1999/6/1

N2 - Twelve Spanish laboratories collected 325 yeast clinical isolates during a 30 day's period, among them 224 Candida albicans, 30 Candida glabrata, and 27 Candida parapsilosis. In vitro antifungal susceptibility to amphotericin B, ketoconazole, fluconazole and itraconazole was determined by an agar diffusion test (Neo-Sensitabs®, Rosco, Denmark). All the isolates tested were susceptible in vitro to amphotericin B and nearly all (97.2%) to itraconazole. In vitro susceptibility to fluconazole and ketoconazole was high (90.2% and 91.4% of isolates, respectively) but showed variations depending on the species tested. Resistance to fluconazole and ketoconazole was low in C. albicans (4% and 3%, respectively), but 30% of Candida guilliermondii and 36% of C. glabrata isolates were resistant to fluconazole. Ketoconazole resistance was observed in 40% of C. glabrata, and 17% of Candida tropicalis. Resistance to antifungal drugs is very low in Spain and it is related to non-C. albicans isolates.

AB - Twelve Spanish laboratories collected 325 yeast clinical isolates during a 30 day's period, among them 224 Candida albicans, 30 Candida glabrata, and 27 Candida parapsilosis. In vitro antifungal susceptibility to amphotericin B, ketoconazole, fluconazole and itraconazole was determined by an agar diffusion test (Neo-Sensitabs®, Rosco, Denmark). All the isolates tested were susceptible in vitro to amphotericin B and nearly all (97.2%) to itraconazole. In vitro susceptibility to fluconazole and ketoconazole was high (90.2% and 91.4% of isolates, respectively) but showed variations depending on the species tested. Resistance to fluconazole and ketoconazole was low in C. albicans (4% and 3%, respectively), but 30% of Candida guilliermondii and 36% of C. glabrata isolates were resistant to fluconazole. Ketoconazole resistance was observed in 40% of C. glabrata, and 17% of Candida tropicalis. Resistance to antifungal drugs is very low in Spain and it is related to non-C. albicans isolates.

KW - Agar diffusion

KW - Antifungal susceptibility

KW - Candida

KW - Cross-resistance

KW - Multicenter

KW - Spain

KW - Yeasts

M3 - Article

SN - 1130-1406

VL - 16

SP - 97

EP - 100

JO - Revista Iberoamericana de Micologia

JF - Revista Iberoamericana de Micologia

IS - 2

ER -

Multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from Spanish patients

Abstract

Keywords

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