Multicenter survey of in vitro antifungal resistance in yeasts of medical importance isolated from Spanish patients

Guillermo Quindós, Lourdes Abarca, Alfonso J. Carrillo-Muñoz, Ma Pilar Arévalo, Fernando J. Bornay, José B. Casals, Juan Manuel Hernández Molina, Isabel Iglesias, Ma José Linares, Estrella Martín-Mazuelos, Manuel Pereiro Ferreirós, Antonio Rezusta, Ma Carmen Rubio, Ricardo Salesa, Rosario San Millán, Josep M. Torres-Rodríguez

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17 Citations (Scopus)


Twelve Spanish laboratories collected 325 yeast clinical isolates during a 30 day's period, among them 224 Candida albicans, 30 Candida glabrata, and 27 Candida parapsilosis. In vitro antifungal susceptibility to amphotericin B, ketoconazole, fluconazole and itraconazole was determined by an agar diffusion test (Neo-Sensitabs®, Rosco, Denmark). All the isolates tested were susceptible in vitro to amphotericin B and nearly all (97.2%) to itraconazole. In vitro susceptibility to fluconazole and ketoconazole was high (90.2% and 91.4% of isolates, respectively) but showed variations depending on the species tested. Resistance to fluconazole and ketoconazole was low in C. albicans (4% and 3%, respectively), but 30% of Candida guilliermondii and 36% of C. glabrata isolates were resistant to fluconazole. Ketoconazole resistance was observed in 40% of C. glabrata, and 17% of Candida tropicalis. Resistance to antifungal drugs is very low in Spain and it is related to non-C. albicans isolates.
Original languageEnglish
Pages (from-to)97-100
JournalRevista Iberoamericana de Micologia
Issue number2
Publication statusPublished - 1 Jun 1999


  • Agar diffusion
  • Antifungal susceptibility
  • Candida
  • Cross-resistance
  • Multicenter
  • Spain
  • Yeasts


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