© 2018 Elsevier Inc. Veterinarians often hold decision-making positions in the public health care system, and therefore can influence public opinion about organ donation. The objective is to analyze the attitude of Spanish veterinarian students toward living liver donation (LLD) and to establish which factors have an influence on this attitude. Methods: A sociological, interdisciplinary, multicenter, and observational study was carried out in the veterinarian students enrolled in Spain (n = 9000) (university academic year 2010–2011). A sample of 2854 students was stratified by geographic area and academic year. A validated questionnaire (PCID-DVH RIOS) was self-administered and completed anonymously. The questionnaire was applied to each academic year at compulsory sessions at randomly selected veterinary schools. Statistical analysis included t test, χ2 test, and logistic regression analysis. Results: Completion rate was 94% (n = 2683); 89% (n = 2345) were in favor of related LLD, and 40% (n = 1053) supported unrelated LLD. The following variables were associated with a more favorable attitude: (1) age (P <.001), (2) sex (P <.001), (3) academic year (P <.001), (4) believing in the possibility of needing a transplant oneself in the future (P <.001), (5) attitude toward deceased donation (P <.001), (6) attitude toward living kidney donation (P <.001), (7) acceptance of a donated liver segment from a family member if one were needed (P <.001), (8) having discussed the subject with one's family (P =.009) and friends (P <.001), (9) a partner's opinion about the subject (P =.002), and (10) fear of the possible mutilation of the body after donation (P <.001). Conclusion: Spanish veterinary students have a favorable attitude toward LLD.