Multicenter evaluation of ATB fungus: A standardized micromethod for yeast susceptibility testing

Guillermo Quindós, Ricardo Salesa, Alfonso J. Carrillo-Muñoz, Verónica Lipperheide, Leopoldo Jáudenes, Rosario San Millán, Josep M. Torres-Rodríguez, José Ponton

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Abstract

The micromethod for yeast susceptibility testing, ATB Fungus, was evaluated with 30 reference strains in three laboratories. Ready-to-use strips with 5-fluorocytosine, amphotericin B, nystatin, miconazole, econazole and ketoconazole were used. The test allowed the categorization of each strain as susceptible, intermediate or resistant to all the antifungals tested, and 5-fluorocytosine and amphotericin B MIC determination. The results were compared with the MIC for each reference strain obtained by a microdilution method on RPMI 1640 buffered with MOPS. The repeatability and intralaboratory and interlaboratory reproducibility were evaluated. ATB Fungus was a reliable and reproducible method with a repeatability of 96.6%, a reproducibility of 95.4% and showed an excellent correlation (91.7%) with reference MICs. © 1994 S. Karger AG, Basel.
Original languageEnglish
Pages (from-to)245-251
JournalChemotherapy
Volume40
Issue number4
DOIs
Publication statusPublished - 1 Jan 1994

Keywords

  • Antifungal susceptibility
  • ATB Fungus
  • Collaborative study

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    Quindós, G., Salesa, R., Carrillo-Muñoz, A. J., Lipperheide, V., Jáudenes, L., Millán, R. S., Torres-Rodríguez, J. M., & Ponton, J. (1994). Multicenter evaluation of ATB fungus: A standardized micromethod for yeast susceptibility testing. Chemotherapy, 40(4), 245-251. https://doi.org/10.1159/000239200