Multi-walled carbon nanotubes (NM401) induce ROS-mediated HPRT mutations in Chinese hamster lung fibroblasts

Laura Rubio, Naouale El Yamani, Alena Kazimirova, Maria Dusinska, Ricard Marcos

Research output: Contribution to journalArticleResearchpeer-review

22 Citations (Scopus)


© 2016 Elsevier Inc. Although there is an important set of data showing potential genotoxic effects of nanomaterials (NMs) at the DNA (comet assay) and chromosome (micronucleus test) levels, few studies have been conducted to analyze their potential mutagenic effects at gene level. We have determined the ability of multi-walled carbon nanotubes (MWCNT, NM401), to induce mutations in the HPRT gene in Chinese hamster lung (V79) fibroblasts. NM401, characterized in the EU NanoGenotox project, were further studied within the EU Framework Programme Seven (FP7) project NANoREG. From the proliferation assay data we selected a dose-range of 0.12 to 12μg/cm2 At these range we have been able to observe significant cellular uptake of MWCNT by using transmission electron microscopy (TEM), as well as a concentration-dependent induction of intracellular reactive oxygen species. In addition, a clear concentration-dependent increase in the induction of HPRT mutations was also observed. Data support a potential genotoxic/ carcinogenic risk associated with MWCNT exposure.
Original languageEnglish
Pages (from-to)185-190
JournalEnvironmental Research
Publication statusPublished - 1 Apr 2016


  • Genotoxicity
  • HPRT gene
  • Multi-walled carbon nanotubes
  • ROS
  • V79 cells


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