Multi-reaction kinetic modeling for the peroxidase–aldolase cascade synthesis of a D-fagomine precursor

Gerard Masdeu*, Zvjezdana Findrik Blažević, Slavko Kralj, Darko Makovec, Josep López-Santín, Gregorio Álvaro

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

2 Citations (Scopus)


The feasibility of a peroxidase–aldolase cascade reaction for the synthesis of therapeutically-valuable iminocyclitols is discussed herein. A two-enzyme system consisting of chloroperoxidase (CPO) and D-fructose-6-phosphate aldolase (FSA) was evaluated for the synthesis of a D-fagomine precursor (preFagomine) from a N-Cbz-3-aminopropanol. An in-depth, systematic, step-by-step kinetic modeling of seven reactions and two inactivation decays was proposed to elucidate the reaction mechanism, prepare suitable stabilized biocatalysts, and find the optimal conditions for its application. The model described accurately the data and predicted the outcome at different experimental conditions. The inactivation of FSA caused by CPO was identified as the main bottleneck in the reaction. A two-step reaction approach and the use of immobilized enzymes on magnetic nanoparticle clusters and functionalized agarose carriers increased the stability of FSA, with an 1839-fold higher preFagomine formation per mol of enzyme in comparison to a one-pot reaction using soluble enzymes.

Original languageEnglish
Article number116602
Number of pages11
JournalChemical Engineering Science
Publication statusPublished - 10 Aug 2021


  • Aldolase
  • D-fagomine
  • Enzymatic reaction cascade
  • Kinetic modeling
  • Peroxidase


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