Multi-approach analysis to assess the chromium(III) immobilization by Ochrobactrum anthropi DE2010

Eduard Villagrasa, Belén Ballesteros, Aleix Obiol, Laia Millach, Isabel Esteve, Antonio Solé

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Ochrobactrum anthropi DE2010 is a microorganism isolated from Ebro Delta microbial mats and able to resist high doses of chromium(III) due to its capacity to tolerate, absorb and accumulate this metal. The effect of this pollutant on O. anthropi DE2010 has been studied assessing changes in viability and biomass, sorption yields and removal efficiencies. Furthermore, and for the first time, its capacity for immobilizing Cr(III) from culture media was tested by a combination of High Angle Annular Dark Field (HAADF) Scanning Transmission Electron Microscopy (STEM) imaging coupled to Energy Dispersive X-ray spectroscopy (EDX).

The results showed that O. anthropi DE2010 was grown optimally at 0-2 mM Cr(III). On the other hand, from 2 to 10 mM Cr(III) microbial plate counts, growth rates, cell viability, and biomass decreased while extracellular polymeric substances (EPS) production increases. Furthermore, this bacterium had a great ability to remove Cr(III) at 10 mM (q = 950.00 mg g(-1)) immobilizing it mostly in bright polyphosphate inclusions and secondarily on the cellular surface at the EPS level. Based on these results, O. anthropi DE2010 could be considered as a potential agent for bioremediation in Cr(III) contaminated environments. (C) 2019 Elsevier Ltd. All rights reserved.

Original languageEnglish
Article number124663
Pages (from-to)124663
Number of pages8
JournalChemosphere
Volume238
DOIs
Publication statusPublished - 1 Jan 2020

Keywords

  • CLSM
  • HAADF-STEM EDX
  • ICP-OES
  • Ochrobactrum anthropi DE2010
  • Polyphosphate inclusions
  • EXTRACELLULAR POLYMERIC SUBSTANCES
  • MECHANISM
  • EPS
  • COPPER
  • HEXAVALENT CHROMIUM
  • BIOACCUMULATION
  • AGRICULTURAL SOILS
  • BIOSORPTION
  • CR(III)
  • LEAD
  • Biodegradation, Environmental
  • Ochrobactrum anthropi/growth & development
  • Biomass
  • Microbial Viability
  • Spectrometry, X-Ray Emission
  • Chromium/metabolism

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