Abstract
Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Original language | English |
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Pages (from-to) | 72-78 |
Journal | FEBS Letters |
Volume | 533 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 2 Jan 2003 |
Keywords
- Cd(II) binding
- Cu(I) binding
- Drosophila metallothionein
- Molecular evolution
- Recombinant synthesis
- Zn(II) binding