MTO: The second member of a Drosophila dual copper-thionein system

J. Domenech; O. Palacios; L. Villarreal; P. González-Duarte; M. Capdevila; S. Atrian

doi:10.1016/S0014-5793(02)03754-7

MTO: The second member of a Drosophila dual copper-thionein system

J. Domenech, O. Palacios, L. Villarreal, P. González-Duarte, M. Capdevila, S. Atrian

Department of Chemistry

Research output: Contribution to journal › Article › Research › peer-review

30 Citations (Scopus)

Abstract

Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	72-78
Journal	FEBS Letters
Volume	533
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(02)03754-7
Publication status	Published - 2 Jan 2003

Keywords

Cd(II) binding
Cu(I) binding
Drosophila metallothionein
Molecular evolution
Recombinant synthesis
Zn(II) binding

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title = "MTO: The second member of a Drosophila dual copper-thionein system",

abstract = "Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates. {\textcopyright} 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.",

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T1 - MTO: The second member of a Drosophila dual copper-thionein system

AU - Domenech, J.

AU - Palacios, O.

AU - Villarreal, L.

AU - González-Duarte, P.

AU - Capdevila, M.

AU - Atrian, S.

PY - 2003/1/2

Y1 - 2003/1/2

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AB - Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

KW - Cd(II) binding

KW - Cu(I) binding

KW - Drosophila metallothionein

KW - Molecular evolution

KW - Recombinant synthesis

KW - Zn(II) binding

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