MTO: The second member of a Drosophila dual copper-thionein system

J. Domenech, O. Palacios, L. Villarreal, P. González-Duarte, M. Capdevila, S. Atrian

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33 Citations (Scopus)


Drosophila MTO metal binding features were analyzed for comparison with MTN, the paralogous Drosophila metallothionein, and to classify MTO as either zinc- or copper-thionein. This was achieved by a combination of in vivo, in vitro and in silico methodologies. All the results unambiguously classified MTO as a second Drosophila copper-thionein, putting Drosophila forward as the only metazoan in which any zinc-thionein has still to be reported. Interestingly, experimental data only showed minor differences in the coordinative behavior of both MTs, but provided a characteristic spectroscopic fingerprint, revealing the possible binding of chloride anions in certain metal-MTO aggregates. © 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
Original languageEnglish
Pages (from-to)72-78
JournalFEBS Letters
Issue number1-3
Publication statusPublished - 2 Jan 2003


  • Cd(II) binding
  • Cu(I) binding
  • Drosophila metallothionein
  • Molecular evolution
  • Recombinant synthesis
  • Zn(II) binding


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