Monolithic PDMS passband filters for fluorescence detection

Andreu Llobera, Stefanie Demming, Haakan N. Joensson, J. Vila-Planas, Helene Andersson-Svahn, Stephanus Büttgenbach

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    Abstract

    We present the fabrication and characteristics of monolithically integrated ink dyed poly(dimethylsiloxane) (PDMS) filters for optical sensing in disposable lab-on-a-chip. This represents a migration of auxillary functions onto the disposable chip with the goal of producing truly portable systems. Filters made from commercially available ink (Pelikan) directly mixed into PDMS oligomer without the use of any additional solvents were patterned with standard soft lithography technologies. Furthermore, a fabrication process based on capillary forces is presented allowing PDMS coloration of arbitrary shapes. Different filters of varying thickness fabricated using red, green and blue ink in four different concentrations were characterized. The optimal performance was found with filter thicknesses of 250 μm and ink to PDMS ratios of 0.1 (mL ink: mL PDMS oligomer) resulting in a transmittance ranging from -15.1 dB to -12.3 dB in the stopband and from -4.0 dB to -2.5 dB in the passband. Additionally, we demonstrate the robustness of this approach as the ink dyed PDMS filters do not exhibit temporal ageing due to diffusion or autofluorescence. We also show that such filters can easily be integrated in fluorescence systems, with stopbands efficient enough to allow fluorescence measurements under non-optimal conditions (broadband excitation, 180° configuration). Integrated ink dyed PDMS filters add robust optical functionalities to disposable microdevices at a low cost and will enable the use of these devices for a wide range of fluorescence and absorbance based biological and chemical analysis. © The Royal Society of Chemistry 2010.
    Original languageEnglish
    Pages (from-to)1987-1992
    JournalLab on a Chip
    Volume10
    Issue number15
    DOIs
    Publication statusPublished - 7 Aug 2010

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  • Cite this

    Llobera, A., Demming, S., Joensson, H. N., Vila-Planas, J., Andersson-Svahn, H., & Büttgenbach, S. (2010). Monolithic PDMS passband filters for fluorescence detection. Lab on a Chip, 10(15), 1987-1992. https://doi.org/10.1039/c003575k