Monoamine oxidase-B activity is not involved in the neuroinflammatory response elicited by a focal freeze brain injury

Elisenda Sanz, Albert Quintana, Tony Valente, Yasmina Manso, Juan Hidalgo, Mercedes Unzeta

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Cryolesion of the frontoparietal cortex in mice is a well-described brain injury paradigm that results in increased astrogliosis surrounding the lesion site and is accompanied by a prominent increase in the MAO-B levels in astrocytes. Whether these increased MAO-B levels contribute to cellular damage or modulate reactive astrocytosis remains unclear. MAO-B activity may contribute to cellular damage, since its metabolism products are highly toxic to the cells. Additionally, it has been suggested that MAO-B inhibition may regulate astrocytic reaction. In this study, we have determined th e relative contribution of MAO-B activity to the outcome following freeze injury. Freeze injury induced a prominent increase of several inflammatory markers, including ICAM, Mac-1, EB22, and GFAP. Inhibition of MAO-B activity using the selective inhibitor PF9601N did not reduce this cryolesion-induced inflammatory response. Additional data revealed that the expression of several cryolesion-induced cell death genes, such as Fas, Rip, p53, and ICE, was not reduced in PF9601N-treated mice, evidencing that MAO-B activity did not contribute to cryolesion-induced cell death. Definitive functional analysis of the mice using the ladder beam task revealed that MAO-B inhibition did not improve the cryolesion-induced motor impairment. These data strongly suggest that, although MAO-B is highly expressed in the area surrounding the lesion site, its activity does not contribute to the cellular damage or play any role in regulating astrocytic reactivity. © 2008 Wiley-Liss, Inc.
Original languageEnglish
Pages (from-to)784-794
JournalJournal of Neuroscience Research
Volume87
Issue number3
DOIs
Publication statusPublished - 15 Feb 2009

Keywords

  • Cell death
  • Cryolesion
  • Inflammation
  • Mice
  • Monoamine oxidase

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