Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis

Burkhard Horstkotte; Carolina Arnau; Francisco Valero; Olaf Elsholz; Víctor Cerdà

doi:10.1016/j.bej.2008.05.020

Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis

Burkhard Horstkotte, Carolina Arnau, Francisco Valero, Olaf Elsholz, Víctor Cerdà

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

A simple and robust analyser system based on sequential injection analysis for the determination of sorbitol in Pichia pastoris MutS phenotype batch fermentation is described. Applicability for the bioprocess monitoring of sorbitol in batch culture medium in a concentration range of 8-1.5 g l-1 with satisfying sorbitol recovery was demonstrated during 60 h. Optimization of physical and chemical parameters is further described. The method proved to be reproducible (R.S.D. < 3%), robust, and fast (17 injections per hour). The SIA sorbitol analyzer showed to be applicable for the monitoring of sorbitol concentration in a mixed substrate P. pastoris fermentation strategy. No interference of methanol was observed. The method is suited for the implementation of sorbitol concentration closed-loop control strategies and for the study of the effect of sorbitol concentration in heterologous protein productivity. © 2008 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	77-83
Journal	Biochemical Engineering Journal
Volume	42
DOIs	https://doi.org/10.1016/j.bej.2008.05.020
Publication status	Published - 15 Oct 2008

Keywords

Batch cultivation
Mixed substrates
Pichia pastoris
Sequential injection analysis
Sorbitol

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10.1016/j.bej.2008.05.020

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title = "Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis",

abstract = "A simple and robust analyser system based on sequential injection analysis for the determination of sorbitol in Pichia pastoris MutS phenotype batch fermentation is described. Applicability for the bioprocess monitoring of sorbitol in batch culture medium in a concentration range of 8-1.5 g l-1 with satisfying sorbitol recovery was demonstrated during 60 h. Optimization of physical and chemical parameters is further described. The method proved to be reproducible (R.S.D. < 3%), robust, and fast (17 injections per hour). The SIA sorbitol analyzer showed to be applicable for the monitoring of sorbitol concentration in a mixed substrate P. pastoris fermentation strategy. No interference of methanol was observed. The method is suited for the implementation of sorbitol concentration closed-loop control strategies and for the study of the effect of sorbitol concentration in heterologous protein productivity. {\textcopyright} 2008 Elsevier B.V. All rights reserved.",

keywords = "Batch cultivation, Mixed substrates, Pichia pastoris, Sequential injection analysis, Sorbitol",

author = "Burkhard Horstkotte and Carolina Arnau and Francisco Valero and Olaf Elsholz and V{\'i}ctor Cerd{\`a}",

year = "2008",

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doi = "10.1016/j.bej.2008.05.020",

language = "English",

volume = "42",

pages = "77--83",

journal = "Biochemical Engineering Journal",

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TY - JOUR

T1 - Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis

AU - Horstkotte, Burkhard

AU - Arnau, Carolina

AU - Valero, Francisco

AU - Elsholz, Olaf

AU - Cerdà, Víctor

PY - 2008/10/15

Y1 - 2008/10/15

N2 - A simple and robust analyser system based on sequential injection analysis for the determination of sorbitol in Pichia pastoris MutS phenotype batch fermentation is described. Applicability for the bioprocess monitoring of sorbitol in batch culture medium in a concentration range of 8-1.5 g l-1 with satisfying sorbitol recovery was demonstrated during 60 h. Optimization of physical and chemical parameters is further described. The method proved to be reproducible (R.S.D. < 3%), robust, and fast (17 injections per hour). The SIA sorbitol analyzer showed to be applicable for the monitoring of sorbitol concentration in a mixed substrate P. pastoris fermentation strategy. No interference of methanol was observed. The method is suited for the implementation of sorbitol concentration closed-loop control strategies and for the study of the effect of sorbitol concentration in heterologous protein productivity. © 2008 Elsevier B.V. All rights reserved.

AB - A simple and robust analyser system based on sequential injection analysis for the determination of sorbitol in Pichia pastoris MutS phenotype batch fermentation is described. Applicability for the bioprocess monitoring of sorbitol in batch culture medium in a concentration range of 8-1.5 g l-1 with satisfying sorbitol recovery was demonstrated during 60 h. Optimization of physical and chemical parameters is further described. The method proved to be reproducible (R.S.D. < 3%), robust, and fast (17 injections per hour). The SIA sorbitol analyzer showed to be applicable for the monitoring of sorbitol concentration in a mixed substrate P. pastoris fermentation strategy. No interference of methanol was observed. The method is suited for the implementation of sorbitol concentration closed-loop control strategies and for the study of the effect of sorbitol concentration in heterologous protein productivity. © 2008 Elsevier B.V. All rights reserved.

KW - Batch cultivation

KW - Mixed substrates

KW - Pichia pastoris

KW - Sequential injection analysis

KW - Sorbitol

U2 - 10.1016/j.bej.2008.05.020

DO - 10.1016/j.bej.2008.05.020

M3 - Article

SN - 1369-703X

VL - 42

SP - 77

EP - 83

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

ER -

Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis

Abstract

Keywords

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