Molecular cytogenetic characterisation of the colorectal cancer cell line SW480

Jordi Camps; Kristin Mrasek; Esther Prat; Anja Weise; Heike Starke; Josep Egozcue; Rosa Miró; Thomas Liehr

Molecular cytogenetic characterisation of the colorectal cancer cell line SW480

Jordi Camps, Kristin Mrasek, Esther Prat, Anja Weise, Heike Starke, Josep Egozcue, Rosa Miró, Thomas Liehr

Research output: Contribution to journal › Article › Research › peer-review

Abstract

It has been demonstrated that 24-color FISH is not sufficient to understand completely the behaviour of chromosomal markers, especially in solid tumors. In the present study we show the usefulness of molecular cytogenetic techniques, such as multicolour banding (MCB) and centromere-specific multicolour-FISH (cenM-FISH) performed on the colorectal cancer cell line SW480. Applying these approaches previously described chromosomal breakpoints could be redefined and six 'marker chromosomes' could be thoroughly characterised. Additionally, the cenM-FISH technique identified three stable dicentric chromosomes which have never been described before in SW480. In conclusion, here we present the first comprehensive characterisation of the complex karyotype of the colorectal cancer cell line SW480.

Original language	English
Pages (from-to)	1215-1218
Journal	Oncology Reports
Volume	11
Issue number	6
Publication status	Published - 1 Jun 2004

Keywords

Colorectal cancer
Multicolour-FISH

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Molecular cytogenetic characterisation of the colorectal cancer cell line SW480",

abstract = "It has been demonstrated that 24-color FISH is not sufficient to understand completely the behaviour of chromosomal markers, especially in solid tumors. In the present study we show the usefulness of molecular cytogenetic techniques, such as multicolour banding (MCB) and centromere-specific multicolour-FISH (cenM-FISH) performed on the colorectal cancer cell line SW480. Applying these approaches previously described chromosomal breakpoints could be redefined and six 'marker chromosomes' could be thoroughly characterised. Additionally, the cenM-FISH technique identified three stable dicentric chromosomes which have never been described before in SW480. In conclusion, here we present the first comprehensive characterisation of the complex karyotype of the colorectal cancer cell line SW480.",

keywords = "Colorectal cancer, Multicolour-FISH",

author = "Jordi Camps and Kristin Mrasek and Esther Prat and Anja Weise and Heike Starke and Josep Egozcue and Rosa Mir{\'o} and Thomas Liehr",

year = "2004",

month = jun,

day = "1",

language = "English",

volume = "11",

pages = "1215--1218",

journal = "Oncology Reports",

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TY - JOUR

T1 - Molecular cytogenetic characterisation of the colorectal cancer cell line SW480

AU - Camps, Jordi

AU - Mrasek, Kristin

AU - Prat, Esther

AU - Weise, Anja

AU - Starke, Heike

AU - Egozcue, Josep

AU - Miró, Rosa

AU - Liehr, Thomas

PY - 2004/6/1

Y1 - 2004/6/1

N2 - It has been demonstrated that 24-color FISH is not sufficient to understand completely the behaviour of chromosomal markers, especially in solid tumors. In the present study we show the usefulness of molecular cytogenetic techniques, such as multicolour banding (MCB) and centromere-specific multicolour-FISH (cenM-FISH) performed on the colorectal cancer cell line SW480. Applying these approaches previously described chromosomal breakpoints could be redefined and six 'marker chromosomes' could be thoroughly characterised. Additionally, the cenM-FISH technique identified three stable dicentric chromosomes which have never been described before in SW480. In conclusion, here we present the first comprehensive characterisation of the complex karyotype of the colorectal cancer cell line SW480.

AB - It has been demonstrated that 24-color FISH is not sufficient to understand completely the behaviour of chromosomal markers, especially in solid tumors. In the present study we show the usefulness of molecular cytogenetic techniques, such as multicolour banding (MCB) and centromere-specific multicolour-FISH (cenM-FISH) performed on the colorectal cancer cell line SW480. Applying these approaches previously described chromosomal breakpoints could be redefined and six 'marker chromosomes' could be thoroughly characterised. Additionally, the cenM-FISH technique identified three stable dicentric chromosomes which have never been described before in SW480. In conclusion, here we present the first comprehensive characterisation of the complex karyotype of the colorectal cancer cell line SW480.

KW - Colorectal cancer

KW - Multicolour-FISH

M3 - Article

SN - 1021-335X

VL - 11

SP - 1215

EP - 1218

JO - Oncology Reports

JF - Oncology Reports

IS - 6

ER -

Molecular cytogenetic characterisation of the colorectal cancer cell line SW480

Abstract

Keywords

UN SDGs

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