Molecular cloning and characterization of two phosphatase 2A catalytic subunit genes from Arabidopsis thaliana

Encarna Pérez-Callejón, Antonio Casamayor, Gemma Pujol, Manel Camps, Albert Ferrer, Joaquín Ariño

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

The plant Arabidopsis thaliana contains five isoforms of the catalytic subunit of protein phosphatase 2A (PP2A) that can be grouped into two families, one composed by isoforms PP2A-1, -2 and -5 and the other composed by isoforms PP2A-3 and PP2A-4. An Arabidopsis genomic library was screened and several clones corresponding to genes PP2A-3 and PP2A-4 were isolated and analysed. Both genes span over approximately 4.5 kbp and are composed of 11 exons and 10 introns that show identical organization. Their untranslated regions are also highly conserved, suggesting that the two genes derive from a common ancestral gene. However, the position of intron/exon junctions completely differs from that of the human PP2A genes. Two transcription start sites have been found in the PP2A-3 gene, the major one mapping at nucleotide position -188 from the translation start codon, whereas only one is observed in PP2A-4 (-145). Functional gene promoter analysis reveals that elements required for transient expression of PP2A-3 and PP2A-4 on a protoplast system are contained within a region of about 600 bp upstream from the transcription start sites. This is the first report on the cloning and characterization of genes encoding catalytic subunits of Ser/Thr protein phosphatases 2A in higher plants.
Original languageEnglish
Pages (from-to)105-112
JournalGene
Volume209
DOIs
Publication statusPublished - 16 Mar 1998

Keywords

  • Gene cloning
  • Promoter analysis
  • Protein dephosphorylation
  • Ser/Thr protein phosphatases

Fingerprint Dive into the research topics of 'Molecular cloning and characterization of two phosphatase 2A catalytic subunit genes from Arabidopsis thaliana'. Together they form a unique fingerprint.

  • Cite this