Molecular characterisation of acquired and overproduced chromosomal bla<inf>AmpC</inf> in Escherichia coli clinical isolates

Noemí Alonso, Elisenda Miró, Vanesa Pascual, Alba Rivera, Maria Simó, Maria Consol Garcia, Mariona Xercavins, Maria Antonia Morera, Elena Espejo, Mercè Gurguí, Josefa Pérez, Mònica Rodríguez-Carballeira, Javier Garau, Esther Calbo, Ferran Navarro, Beatriz Mirelis, Pere Coll

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Abstract

© 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved. Escherichia coli recovered from three hospitals in Barcelona (Spain) were studied to determine the prevalence of isolates with acquired AmpC (ac-AmpC) and/or overproduced chromosomal AmpC (c-AmpC). Mechanisms involved in blac-AmpC overexpression, blaac-AmpC and the plasmids associated with their distribution as well as the prevalence of plasmid-mediated quinolone resistance (PMQR) in AmpC-producing isolates were also determined. Isolates were selected according to their resistance phenotype. blaac-AmpC, alterations in the blac-AmpC promoter/attenuator, and PMQR genes [qnrA, qnrB, qnrS, aac(6′)-Ib-cr and qepA] were characterised by PCR and sequencing. blac-AmpC expression was determined by qRT-PCR. Population structure analysis was performed using PFGE, MLST and phylogenetic group PCR. Plasmids carrying blaac-AmpC were characterised by PCR-based replicon typing and S1-PFGE. IncI1 and IncF plasmids were also analysed by plasmid MLST and replicon sequence typing, respectively. Among 21 563 E. coli isolates, 240 (1.1%) overproduced AmpC β-lactamases, including 180 (75.0%) harbouring ac-AmpC (132 CMY-2 variants and 48 DHA-1) and 60 (25.0%) c-AmpC enzymes. Three mutation profiles in the blac-AmpC promoter/attenuator were associated with a 72.5-, 19.9- and 5.8-fold increased expression, respectively. Moreover, 63.3% of ac-AmpC and 43.3% of c-AmpC isolates belonged to B2, D, E or F phylogenetic groups. PMQR was found in 31% of ac-AmpC isolates [38 qnrB4, 8 aac(6′)-Ib-cr, 6 qnrS1 and 3 qnrB19] and in 10% of c-AmpC isolates [5 aac(6′)-Ib-cr and 1 qnrS1]. IncI1-ST12 and IncF were associated with blaCMY-2 and blaDHA-1, respectively. These results suggest that ac-AmpC β-lactamases were the main mechanism of AmpC production. Isolates and plasmids both showed high genetic diversity.
Original languageEnglish
Pages (from-to)62-68
JournalInternational Journal of Antimicrobial Agents
Volume47
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016

Keywords

  • AmpC promoter
  • Antimicrobial resistance
  • Cephalosporins
  • pMLST
  • PMQR

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    Alonso, N., Miró, E., Pascual, V., Rivera, A., Simó, M., Garcia, M. C., Xercavins, M., Morera, M. A., Espejo, E., Gurguí, M., Pérez, J., Rodríguez-Carballeira, M., Garau, J., Calbo, E., Navarro, F., Mirelis, B., & Coll, P. (2016). Molecular characterisation of acquired and overproduced chromosomal bla<inf>AmpC</inf> in Escherichia coli clinical isolates. International Journal of Antimicrobial Agents, 47(1), 62-68. https://doi.org/10.1016/j.ijantimicag.2015.10.007