Mitomycin C stimulates thermally induced recombinant gene expression in Escherichia coli MC strains

J. L. Corchero; X. Carbonell; A. Benito; A. Villaverde

doi:10.1007/BF00191187

Mitomycin C stimulates thermally induced recombinant gene expression in Escherichia coli MC strains

J. L. Corchero, X. Carbonell, A. Benito, A. Villaverde

Research output: Contribution to journal › Article › Research › peer-review

Abstract

The effects of mitomycin C on C1857-controlled recombinant gene expression have been explored in E. coli cultures when the drug was added simultaneously to the thermal induction. A significantly improved yield of homologous, heterologous and chimeric fusion proteins was observed in E. coli MC1061 and GE864 (a MC4100 derivative) thermoinduced cells. This feature was not detected in other E. coli strains and does not involve a gene dosage mechanism but a strain-dependent stimulation of gene expression unrelated to the RecA protease activity. © 1995 Springer-Verlag.

Original language	English
Pages (from-to)	890-894
Journal	Applied Microbiology and Biotechnology
Volume	42
Issue number	6
DOIs	https://doi.org/10.1007/BF00191187
Publication status	Published - 1 Mar 1995

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title = "Mitomycin C stimulates thermally induced recombinant gene expression in Escherichia coli MC strains",

abstract = "The effects of mitomycin C on C1857-controlled recombinant gene expression have been explored in E. coli cultures when the drug was added simultaneously to the thermal induction. A significantly improved yield of homologous, heterologous and chimeric fusion proteins was observed in E. coli MC1061 and GE864 (a MC4100 derivative) thermoinduced cells. This feature was not detected in other E. coli strains and does not involve a gene dosage mechanism but a strain-dependent stimulation of gene expression unrelated to the RecA protease activity. {\textcopyright} 1995 Springer-Verlag.",

author = "Corchero, {J. L.} and X. Carbonell and A. Benito and A. Villaverde",

year = "1995",

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T1 - Mitomycin C stimulates thermally induced recombinant gene expression in Escherichia coli MC strains

AU - Corchero, J. L.

AU - Carbonell, X.

AU - Benito, A.

AU - Villaverde, A.

PY - 1995/3/1

Y1 - 1995/3/1

N2 - The effects of mitomycin C on C1857-controlled recombinant gene expression have been explored in E. coli cultures when the drug was added simultaneously to the thermal induction. A significantly improved yield of homologous, heterologous and chimeric fusion proteins was observed in E. coli MC1061 and GE864 (a MC4100 derivative) thermoinduced cells. This feature was not detected in other E. coli strains and does not involve a gene dosage mechanism but a strain-dependent stimulation of gene expression unrelated to the RecA protease activity. © 1995 Springer-Verlag.

AB - The effects of mitomycin C on C1857-controlled recombinant gene expression have been explored in E. coli cultures when the drug was added simultaneously to the thermal induction. A significantly improved yield of homologous, heterologous and chimeric fusion proteins was observed in E. coli MC1061 and GE864 (a MC4100 derivative) thermoinduced cells. This feature was not detected in other E. coli strains and does not involve a gene dosage mechanism but a strain-dependent stimulation of gene expression unrelated to the RecA protease activity. © 1995 Springer-Verlag.

U2 - 10.1007/BF00191187

DO - 10.1007/BF00191187

M3 - Article

VL - 42

SP - 890

EP - 894

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 6

ER -