Microglial cell-derived interleukin-6 influences behavior and inflammatory response in the brain following traumatic brain injury

Paula Sanchis, Olaya Fernández-Gayol, Joel Vizueta, Gemma Comes, Carla Canal, Anna Escrig, Amalia Molinero, Mercedes Giralt, Juan Hidalgo*

*Corresponding author for this work

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

Traumatic brain injury (TBI) is a major health problem with high rates of mortality and morbidity worldwide. The response of the brain to TBI is orchestrated by a number of cytokines, including interleukin-6 (IL-6). IL-6 is a major cytokine in the central nervous system and it is produced by different cells, such as neurons, glial cells, and endothelial cells. Since glial cells are one of the most important sources and targets of IL-6, we have examined the role of microglia-derived IL-6 in normal conditions and following a model of TBI, cryolesion of the somatosensorial cortex. To this end, tamoxifen-inducible microglial IL-6-deficient (Il6ΔMic, using Cx3cr1CreER model) mice and control (Il6lox/lox) mice were used. In normal conditions, microglial IL-6 deficiency reduced deambulation and exploratory behavior and decreased anxiety in a sex-dependent manner. The transcriptome profile following cryolesion was dramatically altered 1 day post-lesion in Il6ΔMic compared with Il6lox/lox mice. However, the phenotype of Il6ΔMic mice was less compromised in the following days, suggesting that compensatory mechanisms are at play.

Original languageAmerican English
Pages (from-to)999-1016
Number of pages18
JournalGLIA
Volume68
Issue number5
DOIs
Publication statusPublished - 1 May 2020

Keywords

  • behavior
  • cryolesion
  • knockout microglial IL-6
  • neuroinflammation
  • transcriptome

Fingerprint Dive into the research topics of 'Microglial cell-derived interleukin-6 influences behavior and inflammatory response in the brain following traumatic brain injury'. Together they form a unique fingerprint.

Cite this