In the setting of community-acquired pneumonia (CAP), the conventional microbiological workup is based on culture-based techniques and antigen testing, with a fundamental role in the identification, confirmation and contribution of antibiotic sensitivity of bacterial pathogens involved in its aetiology. However, this is the era of molecular testing and, certainly in past years, novel approaches based on nucleic acid amplification for detection, identification and quantification, as well as sequence-based techniques, have led to important improvements in the diagnosis or exclusion of certain diagnoses, and in the management and monitoring of lower respiratory tract infections. Such approaches attempt to provide accuracy, high sensitivity and specificity, and reduce the turnaround time. Phenotypic methods remain important in the diagnosis of bacterial CAP and molecular approaches, which are increasingly standardised and accessible, are being incorporated more frequently into the routine diagnostic workup. © 2014.
|Journal||European Respiratory Monograph|
|Publication status||Published - 1 Jan 2014|