Microbial strategies for cellulase and xylanase production through solid-state fermentation of digestate from biowaste

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

© 2018 by the authors. Solid-state fermentation (SSF) is a promising technology for producing bioproducts from organic wastes. The objective of this study is to assess the feasibility of using digestate as substrate to produce hydrolytic enzymes, mainly cellulase and xylanase, by exploring three different inoculation strategies: (i) SSF with autochthonous microbiota; (ii) non-sterile SSF inoculated with Trichoderma reesei and (iii) sequential batch operation to select a specialized inoculum, testing two different residence times. Native microbial population did not show a significant cellulase production, suggesting the need for a specialized inoculum. The inoculation of Trichoderma reesei did not improve the enzymatic activity. On the other hand, inconsistent operation was achieved during sequential batch reactor in terms of specific oxygen uptake rate, temperature and enzymatic activity profile. Low cellulase and xylanase activities were attained and the main hypotheses are non-appropriate biomass selection and some degree of hydrolysis by non-targeted proteases produced during fermentation.
Original languageEnglish
Article number2433
JournalSustainability (Switzerland)
Volume10
Issue number7
DOIs
Publication statusPublished - 12 Jul 2018

Keywords

  • Cellulase
  • Digestate
  • Solid-state fermentation
  • Specialized inoculum
  • Xylanase

Fingerprint Dive into the research topics of 'Microbial strategies for cellulase and xylanase production through solid-state fermentation of digestate from biowaste'. Together they form a unique fingerprint.

Cite this