Microbial communities from different types of natural wastewater treatment systems: Vertical and horizontal flow constructed wetlands and biofilters

B. Adrados, O. Sánchez, C. A. Arias, E. Becares, L. Garrido, J. Mas, H. Brix, J. Morató

Research output: Contribution to journalArticleResearchpeer-review

117 Citations (Scopus)

Abstract

The prokaryotic microbial communities (Bacteria and Archaea) of three different systems operating in Denmark for the treatment of domestic wastewater (horizontal flow constructed wetlands (HFCW), vertical flow constructed wetlands (VFCW) and biofilters (BF)) was analysed using endpoint PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE). Further sequencing of the most representative bacterial bands revealed that diverse and distinct bacterial communities were found in each system unit, being γ-Proteobacteria and Bacteroidetes present mainly in all of them, while Firmicutes was observed in HFCW and BF. Members of the Actinobacteria group, although found in HFCW and VFCW, seemed to be more abundant in BF units. Finally, some representatives of α, β and δ-Proteobacteria, Acidobacteria and Chloroflexi were also retrieved from some samples. On the other hand, a lower archaeal diversity was found in comparison with the bacterial population. Cluster analysis of the DGGE bacterial band patterns showed that community structure was related to the design of the treatment system and the organic matter load, while no clear relation was established between the microbial assemblage and the wastewater influent. © 2014 Elsevier Ltd.
Original languageEnglish
Pages (from-to)304-312
JournalWater Research
Volume55
DOIs
Publication statusPublished - 15 May 2014

Keywords

  • Biofilters
  • Constructed wetlands
  • Domestic wastewater
  • Microbial community
  • PCR-DGGE

Fingerprint Dive into the research topics of 'Microbial communities from different types of natural wastewater treatment systems: Vertical and horizontal flow constructed wetlands and biofilters'. Together they form a unique fingerprint.

Cite this