Methanol determination in Pichia pastoris cultures by flow injection analysis

C. Almuzara, O. Cos, M. Baeza, D. Gabriel, F. Valero

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11 Citations (Scopus)

Abstract

An automated reverse flow injection analysis (r-FIA) system using stop-flow technique for quantifying methanol based on the enzymatic reactions of alcohol oxidase and peroxidase was developed. The system permitted methanol analysis in a linear range of 0.006-0.1 g methanol 1-1 without external dilution, and with a sampling frequency of 12 analyses per hour, with a relative standard deviation of 1.16%. The analyser was validated analysing samples from a Pichia pastoris fermentation producing a heterologous protein.
Original languageEnglish
Pages (from-to)413-417
JournalBiotechnology Letters
Volume24
Issue number5
DOIs
Publication statusPublished - 9 Apr 2002

Keywords

  • Alcohol oxidase
  • Flow injection analysis
  • Methanol
  • Peroxidase
  • Pichia pastoris

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