Metabotyping of biofluids reveals stress-based differences in gut permeability in healthy individuals

Serge Rezzi, François Pierre Martin, Carmen Alonso, Mar Guilarte, María Vicario, Laura Ramos, Cristina Martínez, Beatriz Lobo, Esteban Saperas, Juan Ramón Malagelada, Javier Santos, Sunil Kochhar

Research output: Contribution to journalArticleResearchpeer-review

31 Citations (Scopus)


Life stress promotes gut dysfunction, but underlying biochemical events are not well-understood. In the present study, we describe the metabolic events associated with background stress and its potential influence on the response to novel incoming stress stimulus in healthy subjects. A 15 min cold pain test was carried out in healthy men and women stratified according to low (LS; n ) 21) and moderate background stress (MS; n ) 9) during jejunal segmental perfusion. Cold pain induced a stronger psychological response in MS compared to LS subjects, but similar increases in heart rate and blood pressure. Urine and plasma were analyzed using 1H NMR-based metabonomics. Basal stress levels were imprinted in the metabolic profiles indicating different energy and lipid homeostasis. Cold pain increased gut permeability, as determined by mannitol and xylose levels, the response being greater in LS subjects. Moreover, the rate of plasma clearance of mannitol and xylose was dependent on background stress level and gender. In addition, cold pain modulated the levels of circulating ketone bodies, Krebs's cycle intermediates, glucose, and the glucogenic alanine and lactate, which brings further evidence of an alteration of energy homeostasis. Therefore, unravelling life stress with metabonomics may provide a system biology basis for the clinical management and therapeutic surveillance of gut health and disorders. © 2009 American Chemical Society.
Original languageEnglish
Pages (from-to)4799-4809
JournalJournal of Proteome Research
Issue number10
Publication statusPublished - 19 Oct 2009


  • Chemometrics
  • Cold water stress
  • Gut permeability
  • Life stress
  • Metabolomics
  • Metabonomics
  • NMR
  • OPLS


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