Metabolic responses of Quercus ilex seedlings to wounding analysed with nuclear magnetic resonance profiling

J. Sardans, A. Gargallo-Garriga, M. Pérez-Trujillo, T. J. Parella, R. Seco, I. Filella, J. Peñuelas

    Research output: Contribution to journalArticleResearchpeer-review

    18 Citations (Scopus)

    Abstract

    Plants defend themselves against herbivory at several levels. One of these is the synthesis of inducible chemical defences. Using NMR metabolomic techniques, we studied the metabolic changes of plant leaves after a wounding treatment simulating herbivore attack in the Mediterranean sclerophyllous tree Quercus ilex. First, an increase in glucose content was observed in wounded plants. There was also an increase in the content of C-rich secondary metabolites such as quinic acid and quercitol, both related to the shikimic acid pathway and linked to defence against biotic stress. There was also a shift in N-storing amino acids, from leucine and isoleucine to asparagine and choline. The observed higher content of asparagine is related to the higher content of choline through serine that was proved to be the precursor of choline. Choline is a general anti-herbivore and pathogen deterrent. The study shows the rapid metabolic response of Q. ilex in defending its leaves, based on a rapid increase in the production of quinic acid, quercitol and choline. The results also confirm the suitability of 1H NMR-based metabolomic profiling studies to detect global metabolome shifts after wounding stress in tree leaves, and therefore its suitability in ecometabolomic studies. © 2013 German Botanical Society and The Royal Botanical Society of the Netherlands.
    Original languageEnglish
    Pages (from-to)395-403
    JournalPlant Biology
    Volume16
    Issue number2
    DOIs
    Publication statusPublished - 1 Mar 2014

    Keywords

    • Asparagine
    • Choline
    • Glucose
    • Herbivory
    • NMR metabolomics
    • Quercitol
    • Quinic acid
    • Wounding

    Fingerprint Dive into the research topics of 'Metabolic responses of Quercus ilex seedlings to wounding analysed with nuclear magnetic resonance profiling'. Together they form a unique fingerprint.

  • Cite this

    Sardans, J., Gargallo-Garriga, A., Pérez-Trujillo, M., Parella, T. J., Seco, R., Filella, I., & Peñuelas, J. (2014). Metabolic responses of Quercus ilex seedlings to wounding analysed with nuclear magnetic resonance profiling. Plant Biology, 16(2), 395-403. https://doi.org/10.1111/plb.12032