Abstract
Objective: To investigate if proton nuclear magnetic resonance (1H NMR) spectroscopy-based metabolic profiling was able to differentiate follicular lymphoma (FL) from diffuse large B-cell lymphoma (DLBCL) and to study which metabolites were responsible for the differences. Materials and Methods: High-resolution 1H NMR spectra was obtained from fresh samples of lymph node biopsies obtained consecutively at one center (14 FL and 17 DLBCL). Spectra were processed using pattern-recognition methods. Results: Discriminant models were able to differentiate between the two tumor types with a 86% sensitivity and a 76% specificity; the metabolites that most contributed to the discrimination were a relative increase of alanine in the case of DLBCL and a relative increase of taurine in FL. Metabolic models had a significant but weak correlation with Ki67 expression (r2 = 0.42; p = 0.002). Conclusions: We have proved that it is possible to differentiate between FL and DLBCL based on their NMR metabolic profiles. This approach may potentially be applicable as a noninvasive tool for diagnostic and treatment follow-up in the clinical setting using conventional magnetic resonance systems.
Original language | American English |
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Pages (from-to) | 1259-1265 |
Number of pages | 7 |
Journal | Experimental Hematology |
Volume | 37 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2009 |