Metabolic adaptation of colonic microbiota to galactooligosaccharides: a proof-of-concept-study

M. Mego, C. Manichanh, A. Accarino, D. Campos, M. Pozuelo, E. Varela, J. Vulevic, G. Tzortzis, G. Gibson, F. Guarner, F. Azpiroz

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Abstract

© 2017 John Wiley & Sons Ltd Background: Prebiotics have been shown to reduce abdominal symptoms in patients with functional gut disorders, despite that they are fermented by colonic bacteria and may induce gas-related symptoms. Aim: To investigate changes in the metabolic activity of gut microbiota induced by a recognised prebiotic. Methods: Healthy subjects (n = 20) were given a prebiotic (2.8 g/day HOST-G904, HOST Therabiomics, Jersey, Channel Islands) for 3 weeks. During 3-day periods immediately before, at the beginning and at the end of the administration subjects were put on a standard diet (low fibre diet supplemented with one portion of high fibre foods) and the following outcomes were measured: (i) number of daytime gas evacuations for 2 days by means of an event marker; (ii) volume of gas evacuated via a rectal tube during 4 h after a test meal; and (iii) microbiota composition by faecal Illumina MiSeq sequencing. Results: At the beginning of administration, HOST-G904 significantly increased the number of daily anal gas evacuations (18 ± 2 vs. 12 ± 1 pre-administration; P < 0.001) and the volume of gas evacuated after the test meal (236 ± 23 mL vs. 160 ± 17 mL pre-administration; P = 0.006). However, after 3 weeks of administration, these effects diminished (11 ± 2 daily evacuations, 169 ± 23 mL gas evacuation). At day 21, relative abundance of butyrate producers (Lachnospiraceae) correlated inversely with the volume of gas evacuated (r = −0.52; P = 0.02). Conclusion: The availability of substrates induces an adaptation of the colonic microbiota activity in bacterial metabolism, which produces less gas and associated issues. Clinical trials.gov NCT02618239.
Original languageEnglish
Pages (from-to)670-680
JournalAlimentary Pharmacology and Therapeutics
Volume45
Issue number5
DOIs
Publication statusPublished - 1 Mar 2017

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    Mego, M., Manichanh, C., Accarino, A., Campos, D., Pozuelo, M., Varela, E., Vulevic, J., Tzortzis, G., Gibson, G., Guarner, F., & Azpiroz, F. (2017). Metabolic adaptation of colonic microbiota to galactooligosaccharides: a proof-of-concept-study. Alimentary Pharmacology and Therapeutics, 45(5), 670-680. https://doi.org/10.1111/apt.13931