TY - JOUR
T1 - Mechanistics of trichloroethylene mineralization by the white-rot fungus Trametes versicolor
AU - Marco-Urrea, Ernest
AU - Parella, Teodor
AU - Gabarrell, Xavier
AU - Caminal, Gloria
AU - Vicent, Teresa
AU - Adinarayana Reddy, C.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - The white-rot fungus Trametes versicolor degraded trichloroethylene (TCE), a highly oxidized chloroethene, and produced 2,2,2-trichloroethanol and carbon dioxide as the main products of degradation, based on the results obtained using [13C]-TCE as the substrate. For a range of concentrations of TCE between 2 and 20 mg l-1, 53% of the theoretical maximum chloride expected from complete degradation of TCE was observed. Laccase was shown to be induced by TCE, but did not appear to play a role in TCE degradation. Cytochrome P-450 appears to be involved in TCE degradation, as evidenced by marked inhibition of degradation of TCE in the presence of 1-aminobenzotriazole, a known inhibitor of cytochrome P-450. Our results suggested that chloral (trichloroacetaldehyde) was an intermediate of the TCE degradation pathway. The results indicate that the TCE degradation pathway in T. versicolor appears to be similar to that previously reported in mammals and is mechanistically quite different from bacterial TCE degradation. © 2007 Elsevier Ltd. All rights reserved.
AB - The white-rot fungus Trametes versicolor degraded trichloroethylene (TCE), a highly oxidized chloroethene, and produced 2,2,2-trichloroethanol and carbon dioxide as the main products of degradation, based on the results obtained using [13C]-TCE as the substrate. For a range of concentrations of TCE between 2 and 20 mg l-1, 53% of the theoretical maximum chloride expected from complete degradation of TCE was observed. Laccase was shown to be induced by TCE, but did not appear to play a role in TCE degradation. Cytochrome P-450 appears to be involved in TCE degradation, as evidenced by marked inhibition of degradation of TCE in the presence of 1-aminobenzotriazole, a known inhibitor of cytochrome P-450. Our results suggested that chloral (trichloroacetaldehyde) was an intermediate of the TCE degradation pathway. The results indicate that the TCE degradation pathway in T. versicolor appears to be similar to that previously reported in mammals and is mechanistically quite different from bacterial TCE degradation. © 2007 Elsevier Ltd. All rights reserved.
KW - Biodegradation
KW - Cytochrome P-450
KW - Laccase-mediators
KW - TCE degradation pathway
KW - TCE mineralization
KW - Trametes versicolor
U2 - https://doi.org/10.1016/j.chemosphere.2007.06.074
DO - https://doi.org/10.1016/j.chemosphere.2007.06.074
M3 - Article
VL - 70
SP - 404
EP - 410
ER -